Summary of Study ST001210
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000813. The data can be accessed directly via it's Project DOI: 10.21228/M8KX27 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001210 |
Study Title | Comprehensive UHPLC-MS/MS lipidomics profiling to study effects of betulin on keratinocytes |
Study Summary | Lipidomics analysis of betulin in human primary keratinocytes to monitor alterations in the lipid profiles induced by treatment with betulin |
Institute | Eberhard Karls University of Tübingen |
Department | Institute of Pharmaceutical Sciences |
Laboratory | Pharmaceutical (Bio-)Analysis |
Last Name | Calderon |
First Name | Carlos |
Address | Auf der Morgenstelle 8 (Haus B), D-72076, Tuebingen, baden württemberg, 72076, Germany |
carlos.calderon@uni-tuebingen.de | |
Phone | +49 (0)7071 29 74009 |
Submit Date | 2019-07-05 |
Num Groups | 2 |
Total Subjects | 20 |
Study Comments | Eberhard-Karls-University Tuebingen |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2019-09-23 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001292 |
Treatment Summary: | Cells were split when they reached a confluency of 80 %. Passage 3 or 4 were used. Cells were plated in 20 Petri dishes (10 cm2, 1 x 106 cells/ per dish) and cultivated for 4 days in 10 mL of the above mentioned medium for adherence. 10 dishes were incubated with 1.95 µM betulin (10 µL of the 1.95 mM stock solution in DMSO) for 8 hrs prior to removal of the medium and the remaining ones were used as control. Control samples were treated with 10 µL DMSO. The concentration of 1.95 µM of betulin was used, as this concentration has shown effects in our previous studies on the molecular wound healing effect [1]. On day 4, cells were incubated with 3 ml of trypsin (0.05 %) at 37°C. After 5 min cells were washed by adding 7 mL medium. The suspension was transferred into 15 mL falcon tubes and centrifuged for 5 min at 4°C and 1.200 rpm, respectively. The supernatant was withdrawn and the remaining cell pellets were washed and then frozen at -20°C in the falcon tubes until extraction. |
Treatment Protocol Filename: | ccalcas_Extraction_and_treatment.pdf |