Summary of Study ST001219

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000818. The data can be accessed directly via it's Project DOI: 10.21228/M8Z68P This work is supported by NIH grant, U2C- DK119886.

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Study IDST001219
Study TitleVitamin D regulates the microbiota to induce RORgt/FoxP3+ regulatory T cells
Study SummaryThe active form of vitamin D (1,25(OH)2D) suppresses experimental models of inflammatory bowel disease in part by regulating the microbiota. In this study, the role of vitamin D in the regulation of microbe induced RORgt/FoxP3+ T regulatory (reg) cells in the colon was determined. Vitamin D sufficient (D+) mice had significantly higher frequencies of FoxP3+ and RORgt/FoxP3+ T reg cells in the colon compared to vitamin D deficient (D-) mice. The higher frequency of RORgt/FoxP3+ T reg cells in D+ colon correlated with higher numbers of bacteria from the Clostridium XIVa and Bacteroides in D+ compared to D- cecum. D- mice with fewer RORgt/FoxP3+ T reg cells were significantly more susceptible to colitis than D+ mice. Transfer of the cecal bacteria from D+ or D- mice to germfree recipients phenocopied the higher numbers of RORgt/FoxP3+ cells and reduced susceptibility to colitis in D+ versus D- recipient mice. 1,25(OH)2D treatment of the D- mice beginning at 3 weeks of age did not completely recover RORgt/FoxP3+ T reg cells or the Bacteriodes, Bacteriodes thetaiotaomicron, and Clostridium XIVa numbers to D+ values. Early vitamin D status shapes the microbiota to optimize the population of colonic RORgt/FoxP3+ T reg cells important for resistance to colitis.
Institute
Pennsylvania State University
Last NameNichols
First NameRobert
Address917 Old Boalsburg Road, State College, Pennsylvania, 16801, USA
Emailrgn5011@psu.edu
Phone7247662694
Submit Date2019-07-17
Raw Data AvailableYes
Analysis Type DetailNMR
Release Date2019-09-23
Release Version1
Robert Nichols Robert Nichols
https://dx.doi.org/10.21228/M8Z68P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001301
Treatment Summary:C57BL/6 mice were originally from Jackson Labs (Bar Harbor, MN) and maintained at the Pennsylvania State University (University Park, PA). All mice used were housed within the same rooms in the animal facility. Mice were fed purified diets made in the lab as described that either contained vitamin D (D+) or did not (D-) 24. GF C57BL/6 mice were bred and maintained at the Pennsylvania State University gnotobiotic animal research facility. For some experiments, 1,25D was started in the diets of 3-5 week old mice (25ng/d until 5 weeks of age) and mice that were >5 weeks old were fed 50ng/d 1,25D exactly as described 24. For microbial transplantation experiments (two independent experiments), the 4 week old GF mice were gavaged with 100 µl/10 µg cecal contents from D+ and D- mice, and used for experiments after 2 weeks 25. All of the experimental procedures were approved by the Institutional Animal Care and Use Committee at the Pennsylvania State University
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