Summary of Study ST001270

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000855. The data can be accessed directly via it's Project DOI: 10.21228/M85H5H This work is supported by NIH grant, U2C- DK119886.


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Study IDST001270
Study TitleNecrotizing soft-tissue infections (NSTIs) metabolomics
Study SummaryNecrotizing soft-tissue infections (NSTIs) have multiple causes, risk factors, anatomical locations, and pathogenic mechanisms. In patients with NSTI, circulating metabolites may serve as substrate having impact on bacterial adaptation at the site of infection. Metabolic signatures associated with NSTI may reveal potential be useful as diagnostic and prognostic markers, as well as novel targets for therapy. This study used untargeted metabolomics analyses of plasma from NSTI patients (n=34) and healthy (non-infected) controls (n=24) to identify the metabolic signatures and connectivity patterns among metabolites associated with NSTI.
Wageningen University & Research
Last NameEdoardo
First NameSaccenti
AddressStippeneng 4, 6708 Wageningen, the Netherlands
Submit Date2019-10-28
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2020-01-06
Release Version1
Saccenti Edoardo Saccenti Edoardo application/zip

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Treatment ID:TR001353
Treatment Summary:Sample preparation was performed according to A et al (A et al. 2005). In detail, 900 uL of extraction buffer (90/10 v/v methanol:water) including internal standards were added to 100 uL of sample material. The sample was shaken at 30 Hz for 3 minutes in a mixer mill and proteins were precipitated at +4 °C on ice. The sample was centrifuged at +4 °C, 14 000 rpm, for 10 minutes. 200 uL of supernatant were transferred to a micro vial and solvents were evaporated. **** Derivatization was performed according to A et al (A et al. 2005). In detail 30 uL of methoxyamine (15 ug/uL in pyridine) were added to the dry sample and the sample was shaken vigorously for 10 minutes. The reaction was begun by keeping the sample at +70 °C for 1 hour before letting the reaction proceed in room temperature for 16 hours. 30 uL of MSTFA were thenceforth added, the sample was shaken and left to react for 1 hour in room temperature. 30 μL of methyl stearate (15 ng/uL in heptane) were added before analysis. *** REFERENCES A, J., et al. (2005), 'Extraction and GC/MS Analysis of the Human Blood Plasma Metabolome', Anal. Chem., 77 (24), 8086-94.
Treatment Protocol Filename:Analytical_parameters_Sleipner_10m_column_P0287_plasma.pdf