Summary of Study ST001298

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000879. The data can be accessed directly via it's Project DOI: 10.21228/M82M47 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001298
Study TitleMetabolome Profiling of Synechococcus elongatus PCC 11801 strains engineered for Succinate Production
Study TypeMeasurement of relative metabolite pools of wild type and engineered Synechococcus elongatus PCC 11801 strains using Isotopic Ratio Method
Study SummaryExperiments to measure relative metabolite pools of wild type Synechococcus elongatus PCC 11801 and its recombinants producing succinate. The wild type and the engineered strains producing succinate were cultivated at 1% CO2 and their metabolome data was collected in three biological and three technical replicates (n=9). The study aims to find metabolomics changes between the wild type and the engineered to identify potential rate-limiting steps that be used as targets for improved production.
Institute
Indian Institute of Technology Bombay
DepartmentDepartment of Chemical Engineering
LaboratoryBio systems Engineering Lab
Last NameWangikar
First NamePramod P
AddressDepartment of Chemical Engineering, IIT Bombay, Powai, Mumbai, Maharashtra, India - 400076
Emailwangikar@iitb.ac.in
Phone22254215
Submit Date2020-01-03
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2020-07-05
Release Version1
Pramod P Wangikar Pramod P Wangikar
https://dx.doi.org/10.21228/M82M47
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001387
Treatment Summary:The metabolites were extracted using a methanol-chloroform-water method described in the Metabolite Extraction Protocol file of the collection data.
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