Summary of Study ST001302

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000883. The data can be accessed directly via it's Project DOI: 10.21228/M8JM4K This work is supported by NIH grant, U2C- DK119886.

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Study IDST001302
Study TitleMetabolome Profiling of a Fast-growing Cyanobacterium Synechococcus elongatus PCC 11801 under Diurnal Cycle
Study TypeMeasurement of relative metabolite pools under diurnal cycle using isotopic ratio method
Study SummaryExperiments were carried out by growing Synechococcus elongatus PCC 11801 cells in multicultivators designed to provide sinusoidal light. The period was 14:10 (light-dark). The samples for metabolomics analysis were collected in the second diurnal cycle at an interval of 6 hours starting from 25th hour (25, 31, 37 and 43 hours). The light intensity amplitude was 600 µmole photons.m-2. s-1. Additionally samples were also collected using cells grown under continuous light illumination of 600 µmole photons.m-2. s-1 to compare the difference in metabolite levels compared to that in the diurnal cycle.
Institute
Indian Institute of Technology Bombay
DepartmentDepartment of Chemical Engineering
LaboratoryBiosystems Engineering Lab
Last NameWangikar
First NamePramod P
AddressDepartment of Chemical Engineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, India - 400076
Emailwangikar@iitb.ac.in
Phone+91 (22) 2576 7232 (o)
Submit Date2020-01-06
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2020-10-11
Release Version1
Pramod P Wangikar Pramod P Wangikar
https://dx.doi.org/10.21228/M8JM4K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001391
Treatment Summary:The metabolites were extracted using a methanol-chloroform-water method described in the Sample Collection and Treatment Protocol file of the collection data.
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