Summary of Study ST001382
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000946. The data can be accessed directly via it's Project DOI: 10.21228/M8DM63 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001382 |
| Study Title | Distinct metabolic states of a cell guide alternate fates of mutational buffering through altered proteostasis |
| Study Summary | Changes in metabolism can alter the cellular milieu; can this also change intracellular proteostasis? Since proteostasis can modulate mutational buffering, if change in metabolism has the ability to change proteostasis, arguably, it should also alter mutational buffering. Building on this, we find that altered cellular metabolic states in E. coli buffer distinct mutations. Buffered-mutants had folding problems in vivo and were differently chaperoned in different metabolic states. Notably, this assistance was dependent upon the metabolites and not on the increase in canonical chaperone machineries. Additionally, we were able to reconstitute the folding assistance afforded by metabolites in vitro and propose that changes in metabolite concentrations have the potential to alter proteostasis. Collectively, we unravel that the metabolite pools are bona fide members of proteostasis and aid in mutational buffering. Given the plasticity in cellular metabolism, we posit that metabolic alterations may play an important role in the positive or negative regulation of proteostasis. |
| Institute | CSIR-National Chemical Laboratory |
| Last Name | Shanmugam |
| First Name | Dhanasekaran |
| Address | Dr. Homi Bhabha Road, Pune, maharashtra, 411008, India |
| d.shanmugam@ncl.res.in | |
| Phone | 2025902719 |
| Submit Date | 2020-05-14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-06-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR001471 |
| Treatment Summary: | CSH4 and WG350 strains were transformed with the mutant GFP clones and subjected to osmotic stress with 350mM NaCl. The pool of mutants buffered under osmotic stress in WG350 was sorted and single clones of GFP were picked from here and checked for their fluorescence in presence and absence of osmotic stress. |
