Summary of Study ST001396
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000952. The data can be accessed directly via it's Project DOI: 10.21228/M8N397 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001396 |
Study Title | Quantitative Lipids study on total murine liver tissue from mice at different age |
Study Type | Liver tissue/Primary tissue |
Study Summary | Following birth, the neonatal intestine is exposed to maternal and environmental bacteria that successively form a dense and highly dynamic intestinal microbiota. Whereas the effect of exogenous factors has been extensively investigated, endogenous, host-mediated mechanisms have remained largely unexplored. Concomitantly with microbial colonization, the liver undergoes functional transition from a hematopoietic organ to a central organ of metabolic regulation and immune surveillance. The aim of the present study was to analyze the influence of the developing hepatic function and liver metabolism on the early intestinal microbiota. Using metabolomic and microbial profiling in combination with multivariate analysis we characterized the colonization dynamics and liver metabolism in the murine gastrointestinal tract (n=6-10 per age group). We observed major age-dependent microbial and metabolic changes and identified bile acids as potent drivers of the early intestinal microbiota maturation. Consistently, oral administration of tauro-cholic acid or β-tauro-murocholic acid to newborn mice (n= 7-14 per group) accelerated postnatal microbiota maturation. Lipids in total liver tissue from healthy C57BL/6 mice at 1, 7, 14, 21, 28 and 56 day after birth was analyzed. |
Institute | Helmholtz Centre for Environmental Research |
Department | Department of Molecular Systems biology |
Laboratory | Functional Metabolomics |
Last Name | Rolle-Kampczyk |
First Name | Ulrike |
Address | Permoserstrasse 15, 04318 Leipzig, Germany |
ulrike.rolle-kampczyk@ufz.de | |
Phone | 0049 341 235 1537 |
Submit Date | 2020-06-03 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2020-06-10 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR001485 |
Treatment Summary: | All C57BL6J wildtype mice were bred locally and held under specific pathogen-free conditions at the Institute of Laboratory Animal Science at RWTH Aachen University Hospital. The day of birth was considered day 0, i.e. animals screened at day 1 were approximately 24 h old and verified to have ingested breast milk (abdominal milk spot). Mice were weaned at PND21. |