Summary of Study ST001490

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001007. The data can be accessed directly via it's Project DOI: 10.21228/M8J106 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001490
Study Title	Plasma lipidomic profiles after a low and high glycemic load dietary pattern in a randomized controlled cross over feeding study
Study Summary	Background: Dietary patterns low in glycemic load are associated with reduced risk of cardiometabolic diseases. Improvements in serum lipid concentrations may play a role in these observed associations. Objective: We investigated how dietary patterns differing in glycemic load affect a clinical lipid panel and plasma lipidomics profiles. Methods: In a crossover, controlled feeding study, 80 healthy participants (n=40 men, n=40 women), 18-45 y were randomized to receive low-glycemic load (LGL) or high glycemic load (HGL) diets for 28 days each with at least a 28-day washout period between controlled diets. Fasting plasma samples were collected at baseline and end of each diet period. A clinical lipid panel including total-, VLDL-, LDL-, and HDL-cholesterol and triglycerides were measured using an auto-analyzer. Lipidomics analysis using mass-spectrometry provided the concentrations of 863 species. Linear mixed models were used to test for a diet effect. Results: Lipids from the clinical panel were not significantly different between diets. Lipidomics analysis showed that 67 lipid species, predominantly in the triacylglycerol class, differed between diets (FDR<0.05). A majority of these were higher after the LGL diet compared to the HGL. Conclusion: While the clinical lipid measures did not differ between diets, some lipid species were higher after the LGL diet in the lipidomics analysis. The two diets were eucaloric and had similar percentage of energy from carbohydrate, protein and fat. Thus, the difference in macronutrient, particularly carbohydrate, quality of the LGL diet is likely affecting the composition of lipid species.
Institute	Fred Hutchinson Cancer Research Center
Last Name	Dibay Moghadam
First Name	Sepideh
Address	1100 Fairview Ave N, Seattle, WA 98109
Email	sdibaymo@fredhutch.org
Phone	206-667-4068
Submit Date	2020-09-10
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	FIA-MS
Release Date	2020-09-24
Release Version	1

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Treatment:

Treatment ID:	TR001579
Treatment Summary:	A 7-day rotating menu was created for each diet. At baseline, participants completed a 3-d food record to estimate mean daily calorie intake. Energy intake from the food records along with weight, height, sex and activity level were used to estimate each participant’s daily energy needs necessary to maintain the current weight. The estimated calorie intake was used to adjust the 7-day rotating menu to meet each participant’s needs so that they would remain weight stable during the study. The percentage energy from macronutrients of the two diets were identical: 15% energy from protein, 30% energy from fat, and 55% energy from carbohydrate. The LGL diet provided on average 55 g/d of fiber and 77 g/d of fructose, with a GL of 125 (Table 1). The HGL diet substituted refined grains for whole grains, included other carbohydrates from high-glycemic index food sources and provided on average 28 g/d of fiber and 26 g/d of fructose, with a GL of 250. All food was prepared and provided by the Fred Hutch Human Nutrition Laboratory (HNL) during the intervention. Weekday dinners were consumed under supervision at the HNL, and the next day’s breakfast, lunch and snacks were portioned, packaged and taken home for consumption. Examples of study menus and detail on diet consumption have been published previously (Neuhouser et al. 2012).

Treatment ID:

TR001579

Treatment Summary:

A 7-day rotating menu was created for each diet. At baseline, participants completed a 3-d food record to estimate mean daily calorie intake. Energy intake from the food records along with weight, height, sex and activity level were used to estimate each participant’s daily energy needs necessary to maintain the current weight. The estimated calorie intake was used to adjust the 7-day rotating menu to meet each participant’s needs so that they would remain weight stable during the study. The percentage energy from macronutrients of the two diets were identical: 15% energy from protein, 30% energy from fat, and 55% energy from carbohydrate. The LGL diet provided on average 55 g/d of fiber and 77 g/d of fructose, with a GL of 125 (Table 1). The HGL diet substituted refined grains for whole grains, included other carbohydrates from high-glycemic index food sources and provided on average 28 g/d of fiber and 26 g/d of fructose, with a GL of 250. All food was prepared and provided by the Fred Hutch Human Nutrition Laboratory (HNL) during the intervention. Weekday dinners were consumed under supervision at the HNL, and the next day’s breakfast, lunch and snacks were portioned, packaged and taken home for consumption. Examples of study menus and detail on diet consumption have been published previously (Neuhouser et al. 2012).