Summary of Study ST001496

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001014. The data can be accessed directly via it's Project DOI: 10.21228/M8MT3W This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001496
Study Title	Application of developed quantatation method
Study Summary	To apply this method in a pilot study, we spiked either taurine or sulfoquinovose into an in vitro simplified human microbiota model with and without Bilophila wadsworthia, a known sulfonate utilizer.
Institute	Helmholtz Centre for Environmental Research
Last Name	Haange
First Name	Sven
Address	Permoserstrasse 1, Leipzig, Saxony, 04318, Germany
Email	sven.haange@ufz.de
Phone	0049 341 2351099
Submit Date	2020-10-02
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2020-10-28
Release Version	1

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Treatment:

Treatment ID:	TR001585
Treatment Summary:	For each 10 ml incubation, DS medium supplemented with either taurine (20 mM) or sulfoquinovose (SQ, 4 mM) was inoculated with the SIHUMI bacteria or the SIHUMI consortium and B. wadsworthia. Control incubations of medium containing only sulfonates or bacteria were included. The incubations were conducted under anoxic conditions at 37 °C in duplicates. Samples (600 µl) were withdrawn after 0, 3, 24 and 48 h for quantification of sulfoquinovose (SQ), 2,3-dihydroxypropane-1-sulfonate (DHPS), taurine and isethionate.

Treatment ID:

TR001585

Treatment Summary:

For each 10 ml incubation, DS medium supplemented with either taurine (20 mM) or sulfoquinovose (SQ, 4 mM) was inoculated with the SIHUMI bacteria or the SIHUMI consortium and B. wadsworthia. Control incubations of medium containing only sulfonates or bacteria were included. The incubations were conducted under anoxic conditions at 37 °C in duplicates. Samples (600 µl) were withdrawn after 0, 3, 24 and 48 h for quantification of sulfoquinovose (SQ), 2,3-dihydroxypropane-1-sulfonate (DHPS), taurine and isethionate.