Summary of Study ST001507
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001017. The data can be accessed directly via it's Project DOI: 10.21228/M87M4S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001507 |
| Study Title | Hepatic [U-13C]Lactate tracing and metabolomics in young and old WT and SIRT6 overexpressing mice |
| Study Summary | Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression. Since the liver is the main gluconeogenic organ, we performed here liver metabolomics in young (6 months) versus old (20-24 months) WT and SIRT6-transgenic mice. We used liver tissues of 6h morning-fasted mice, either in naïve state or 15 minutes after intraperitoneal injection of 1mg/kg [U-13C]Lactate. |
| Institute | Bar Ilan University |
| Department | The Mina & Everard Goodman Faculty of Life Sciences |
| Laboratory | Laboratory of Prof. Haim Cohen |
| Last Name | Cohen |
| First Name | Chaim |
| Address | Bar Ilan University, Ramat Gan, Israel, 5290002 |
| Haim.Cohen@biu.ac.il | |
| Phone | +97235318383 |
| Submit Date | 2020-10-06 |
| Num Groups | 8 |
| Total Subjects | 40 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-03-11 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR001596 |
| Treatment Summary: | All mice were fasted for 6h (8AM-2PM). Before sacrifice, tail blood was collected from all mice for less than 2 minutes. The untreated group were sacrificed at this point. For 13C-lactate treatment, 6h fasted mice were intraperitoneally injected with 1mg/kg [U-13C]Lactate (CIL) and sacrificed 15 minutes after injection. |
| Treatment: | [U-13C]Lactate injection |
| Treatment Compound: | [U-13C]Lactate |
| Treatment Route: | Intraperitoneal injection |
| Treatment Dose: | 1mg/kg |
| Treatment Dosevolume: | 300ul for 30gr mouse |
| Animal Anesthesia: | N/A |
| Animal Fasting: | 6h morning fast |
| Animal Endp Euthanasia: | Cervical dislocation |
