Summary of Study ST001633
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001031. The data can be accessed directly via it's Project DOI: 10.21228/M8F39C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001633 |
| Study Title | Aromatic amino acid metabolism by the anaerobic gut bacterium Clostridium sporogenes (part-II) |
| Study Summary | Germ-free MCAD-/- mice or MCAD+/+ controls were mono-colonized with wild-type Clostridium sporogenes. Urine was collected for untargeted LC-QTOF analysis. |
| Institute | Stanford University |
| Laboratory | Dodd |
| Last Name | Pruss |
| First Name | Kali |
| Address | 300 Pasteur Drive, Lane 235 |
| kmpruss@stanford.edu | |
| Phone | 6507212961 |
| Submit Date | 2020-12-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-01-26 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR001723 |
| Treatment Summary: | MCAD-/- mice were re-derived as germ-free (GF) for the purpose of this study. MCAD-/- mice or MCAD+/+ controls were maintained in sterile gnotobiotic isolators or cages with HEPA-filtered air supply. Urine from GF MCAD-/- and MCAD+/+ mice was collected under sterile conditions. Seven days subsequent, mice were mono-colonized with wild-type C. sporogenes (Cs) by gavage of 200 µL saturated overnight culture. Urine was collected one week after colonization. C. sporogenes colonization was verified with serial dilution plating. |
