Summary of Study ST001829

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001156. The data can be accessed directly via it's Project DOI: 10.21228/M88T3S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001829
Study Title	Analysis and annotation of oxidized PCs generated in vitro.
Study Summary	Oxidized PC16:0/PUFA (18:2, 20:4 and 22:6) generated in vitro were analyzed by LC/HRMS/MS analysis.All mass spectrometry raw data obtained in this study were deposited.
Institute	Kyushu university
Last Name	Matsuoka
First Name	Yuta
Address	3-1-1 Maidashi Higashi-ku
Email	ymatsu1205@gmail.com
Phone	+81-92-642-6624
Submit Date	2021-06-13
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2021-10-14
Release Version	1

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Treatment:

Treatment ID:	TR001919
Treatment Summary:	For in vitro experiments, a mixture containing 500 µM PC16:0/PUFAs (PC16:0/18:2, PC16:0/20:4, and PC16:0/22:6), 0.5% ethanol, and either 50 mM AAPH or 50 mM AAPH + 10 µM hemin was incubated in phosphate-buffered saline (PBS; pH 7.4) at 37 °C under normal or 18O2 air. For autoxidation, a solution of 500 nmol of PC16:0/18:2 in chloroform (1 mL) was immediately dried using nitrogen gas in a glass tube. The dried samples were incubated at 37 °C for 24 h and then resuspended in methanol (200 µL). For CuSO4 + AsA–induced peroxidation, a solution containing 500 µM PC16:0/18:2 and 500 µM CuSO4 + 1 mM AsA was incubated in PBS (pH 7.4) at 37 °C for 72 h.

Treatment ID:

TR001919

Treatment Summary:

For in vitro experiments, a mixture containing 500 µM PC16:0/PUFAs (PC16:0/18:2, PC16:0/20:4, and PC16:0/22:6), 0.5% ethanol, and either 50 mM AAPH or 50 mM AAPH + 10 µM hemin was incubated in phosphate-buffered saline (PBS; pH 7.4) at 37 °C under normal or 18O2 air. For autoxidation, a solution of 500 nmol of PC16:0/18:2 in chloroform (1 mL) was immediately dried using nitrogen gas in a glass tube. The dried samples were incubated at 37 °C for 24 h and then resuspended in methanol (200 µL). For CuSO4 + AsA–induced peroxidation, a solution containing 500 µM PC16:0/18:2 and 500 µM CuSO4 + 1 mM AsA was incubated in PBS (pH 7.4) at 37 °C for 72 h.