Summary of Study ST002024
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001286. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002024 |
| Study Title | Plasmodium falciparum stable-isotope carbon labeling to explore metabolic consequences of keto–acid dehydrogenase disruption |
| Study Summary | Plasmodium falciparum cells in culture were treated with respective universally labelled carbon-13 metabolites for 2.5 hours at standard culture concentrations (glucose or glutamine) or 5 mM (acetate). Metabolites were isolated using 90% methanol, dried, reconstituted in HPLC-grade water, and analyzed by HPLC/MS. Resulting data were analyzed and compiled to generate study data. |
| Institute | Pennsylvania State University |
| Department | Chemistry |
| Laboratory | Llinás Laboratory |
| Last Name | Llinás |
| First Name | Manuel |
| Address | W126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA |
| mul27@psu.edu | |
| Phone | 814-867-3444 |
| Submit Date | 2021-07-15 |
| Num Groups | 16 |
| Total Subjects | 146 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-10-03 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR002118 |
| Treatment Summary: | Plasmodium falciparum NF54 attB parasites were cultured in RPMI 1640 medium and magnetically enriched to increase the infected to uninfected RBC ratio. Following hemocytometer counts, 1x10^8 parasites were measured per condition into 5 mL of total media for 2.5 hours in the presence of the carbon-13 metabolite (glucose, glutamine, or acetate). Wild-type or WT represents control parasites without additional alterations. Blanks are sample tubes that follow the same procedures as samples following the quenching of metabolism. Both Pool and QC samples are combined samples of all samples from the analytical batch on that particular day. Dates in YYYYMMDD format are appended to individual samples to indicate the batch in which they were processed. |
| Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017.pdf |
| Treatment Compound: | Universally labelled glucose, glutamine, or acetate |
| Treatment Route: | Transfer to media by pipette |
| Treatment Dosevolume: | 50 uL (glucose), 25 uL (glutamine), or 10 uL (acetate) |
| Treatment Doseduration: | 2.5 hours |
| Treatment Vehicle: | RPMI 1640 media (lacking the respective metabolite) |
| Cell Storage: | Temperature and gas composition controlled incubator |
| Cell Growth Container: | 6-well plate |
| Cell Growth Config: | 5 mL in each sample well, 1x10^8 infected red blood cells per well, conditions performed in triplicate |
| Cell Media: | RPMI 1640 containing Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
