Summary of Study ST002058

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001303. The data can be accessed directly via it's Project DOI: 10.21228/M89135 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002058
Study TitleMuscle/Lung/Tumor metabolomics
Study SummarySkeletal muscle (SkM, tibialis anterior and upper arm) and lung samples analyzed by LC-MS metabolomics. These tissue types are analyzed as healthy control, Mcherry+ tumor, or adjacent tissue.
Institute
University of Colorado Anschutz Medical Campus
Last NameNemkov
First NameTravis
Address12801 East 17th Ave. Research 1 South Rm 9121 Aurora CO 80045
Emailtravis.nemkov@cuanschutz.edu
Phone303-724-3253
Submit Date2022-01-12
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-02-14
Release Version1
Travis Nemkov Travis Nemkov
https://dx.doi.org/10.21228/M89135
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002152
Treatment Summary:Lung and skeletal muscle (TA and bulk muscles from the upper arm) were collected upon necropsy and imaged for mCherry+ 4T1-SkM lesions (Zeiss AxioZoom). Once a mCherry+ lesion was identified, the region was excised, leaving as little tissue margins as possible. The sample was weighed and then flash frozen using LN2. Healthy lung and skeletal muscle samples were collected through the same process, without the mCherry imaging step. 4T1-parental and 4T1-SkM cells were plated in 6-well plates at an initial density of 50,000 cells/well and collected 48h later (final density ~275,000 cells/well). Wells were washed twice with 1x PBS and cells were scraped into 1.5 mL microcentrifuge tubes and spun at 16,000 x g for 3 minutes. Samples were then flash frozen and stored at -80°C until ready to process.
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