Summary of Study ST002116

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001341. The data can be accessed directly via it's Project DOI: 10.21228/M8CM41 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002116
Study TitleComparative NMR metabolomics of the responses of A2780 human ovarian cancer cells to clinically established Pt based drugs
Study SummaryPt based drugs play a very important role in current cancer treatment; yet their cellular and mechanistic aspects are not fully understood. NMR metabolomics provides a powerful tool to investigate the metabolic perturbations induced by Pt drugs in cancer cells and decipher their meaning in relation to the presumed molecular mechanisms. We have carried out a systematic and comparative NMR metabolomics study to analyze the responses of A2780 human ovarian cancer cells to the main clinically established Pt drugs, i.e. cisplatin, carboplatin and oxaliplatin, with a particular attention for the oxaliplatin/cisplatin comparison in view of recently described mechanistic differences. Notably, NMR analysis revealed some moderate and consistent changes in the metabolomic profiles of A2780 cells treated with the 3 Pt drugs with respect to controls but only very small differences among them. Beyond the expected alterations at the level of the nucleic acids the observed changes highlight in all cases induction of a significant ER stress. Owing to the clinical relevance of platinum resistance the behavior of a cisplatin resistant A2780 cancer cell line upon cisplatin treatment was also evaluated.
Institute
University of Florence
DepartmentDepartment of Chemistry
LaboratoryMetabolomics
Last NameGhini
First NameVeronica
Addressvia Luigi Sacconi 6
Emailturano@cerm.unifi.it
Phone+390554574266
Submit Date2022-03-22
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2022-10-03
Release Version1
Veronica Ghini Veronica Ghini
https://dx.doi.org/10.21228/M8CM41
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002213
Treatment Summary:For drug treatment, 2.7 *10^6 A2780 cells were seeded in p100 plate and after 24 hours were exposed to a concentration of the three Pt-drugs, i.e. CIS, OXA and CARBO equal to their 72 h-exposure IC50 value (2.5, 55 and 0.46 uM, respectively). The drug incubations were stopped at two different time points, 24 h and 48 h. The A2780cp cells were exposed to a concentration of cisplatin equal to their 72 h-exposure IC50 value (15 uM). The drug incubation was stopped after 48 h of treatment.
Treatment:Cisplatin, carboplatin and oxaliplatin
Cell Media:RPMI1640
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