Summary of Study ST002352

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002352
Study TitleBiomolecular condensates create phospholipid-enriched microenvironments (Part 2)
Study TypeMetabolomes of in vitro synthesized condensates
Study SummaryProteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1.
Institute
Cornell University
DepartmentDepartment of Pharmacology
LaboratoryDr. Samie Jaffrey
Last NameDumelie
First NameJason
Address1300 York Ave, LC-524, New York City, NY
Emailjdumes98@gmail.com
Phone6465690174
Submit Date2022-11-15
Raw Data AvailableYes
Raw Data File Type(s)mzdata.xml
Analysis Type DetailLC-MS
Release Date2023-03-01
Release Version2
Jason Dumelie Jason Dumelie
https://dx.doi.org/10.21228/M8N71K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002604
Treatment Summary:Mouse liver metabolites were combined with either the condensate-forming low-complexity domains of HNRNPA1, MED1 or full-length SARS-CoV-2 nucleocapsid. Condensates were stimulated with either 0 nM, 150 nM or 600 nM RNA. Condensates were centrifuged to the bottom of a 600 ul tube. Equal fractions from the input sample, aqueous phase and condensate phases were collected separately. Metabolites were extracted from each fraction. Alternatively, liver metabolites were added 10 min after stimulating condensate formation with phage RNA and incubating for 2 min prior to centrifugation. In a separate set of experiments, the extraction procedure from the three fractions (ie aqueous, condensate and input) was altered to remove a heat step.
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