Summary of Study ST002457
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001585. The data can be accessed directly via it's Project DOI: 10.21228/M8TD8H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002457 |
Study Title | Mouse kidney metabolomics (Whole kidney) |
Study Type | MS quantitative analysis |
Study Summary | Here, we reveal for the first time the misregulated metabolic pathways in TSC kidneys and their relevance to TSC-associated cytogenesis. To this end, we have analyzed the metabolic profile of the whole kidney as well as sorted proximal tubule cell (PTCs) extracts. The metabolomics data show that Tsc1 deletion in nephron progenitor cells changes the arginine biosynthesis pathway as well as a substantial number of metabolic pathways. |
Institute | Hadassah Medical Center |
Last Name | Ben-Dov |
First Name | Iddo |
Address | Hadassah Medical Center, Jerusalem, Israel 91120 |
iddo@hadassah.org.il | |
Phone | +97226776881 |
Submit Date | 2023-01-30 |
Num Groups | 3 |
Total Subjects | 26 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-02-26 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002559 |
Treatment Summary: | A mixture of six labeled internal standards was added to the extraction solution for quality control (13C6-Glucose, 13C5-Glutamine, 13C5-Glutamate, 13C1-Alanine, 13C3-Pyruvate and 13C3-Lactate). The exact volume at each tube was adjusted according to tissue weight (average volume of 200 µl). The samples were homogenized using Precellys 24 tissue homogenizer (Bertin Technologies) precooled to 4°C (3 cycles × 30 s at 6000 rpm, with a 30 s gap between each cycle) and later centrifuged at 18,000 g for 15 min at 4°C. The supernatants were transferred into HPLC glass vials and kept at −80°C prior to LC-MS analysis. |