Summary of Study ST002465

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001468. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT5R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002465
Study TitleMap of microbially induced metabolic changes across diverse body sites in mice - Mouse Data
Study SummaryTissue samples from contents along the intestinal tract and systemic sites in mice that did not have any bacteria (germ free) or colonized with a simplified microbiota or more complex microbiota.
Institute
University of Calgary
DepartmentPhysiology and Pharmacology
LaboratoryMcCoy
Last NameBrown
First NameKirsty
Address3330 Hospital Dr NW
Emailkirsty.brown12@gmail.com; kathy.mccoy@ucalgary.ca
Phone2508692232
Submit Date2023-02-04
Total Subjects72
Num Males36
Num Females36
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-02-05
Release Version1
Kirsty Brown Kirsty Brown
https://dx.doi.org/10.21228/M8ZT5R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002567
Treatment Summary:GF and OMM12-colonized gnotobiotic mice were housed in flexible-film isolators within the International Microbiome Centre (IMC) and SPF mice were maintained in the Mouse Barrier Unit (MBU) at the University of Calgary. GF mice were routinely tested for the absence of bacteria by aerobic and anaerobic culture, gram staining, and vital dye (DNA-dye Sytox green) staining of caecal contents and all mice were routinely screened for the presence of pathogens. For SPF colonization by co-housing, 8-week-old GF animals were exported, transferred into sterile individually ventilated cages and transported from the IMC to the MBU where a colonizer SPF mouse was transferred into each cage. For OMM12 colonization by co-housing, 8-week-old GF animals were transferred into isolators containing OMM12-colonized mice and an OMM12-colonized mouse was added to each cage. All animals were fed identical autoclaved diets and maintained with 12-hour light-dark cycle.
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