Summary of Study ST003328

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002070. The data can be accessed directly via it's Project DOI: 10.21228/M81Z4C This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003328
Study Title	Increased Cholesterol Synthesis Drives Neurotoxicity in Patient Stem Cell-Derived Model of Multiple Sclerosis - cellular lipidomics
Study Summary	Lipidomics analysis was performed on directly induced neural stem/progenitor cell (iNSC) lines derived from fibroblasts of patients with progressive multiple sclerosis (PMS) versus age matched controls (AMC) treated or untreated with cholesterol synthesis inhibitor simvastatin.
Institute	University of Colorado Denver
Last Name	Haines
First Name	Julie
Address	12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA
Email	julie.haines@cuanschutz.edu
Phone	3037243339
Submit Date	2024-07-17
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-08-08
Release Version	1

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Treatment:

Treatment ID:	TR003458
Treatment Summary:	iNSCs were seeded in NIM with Y-27632 (10 µM) at a density of 100,000 cells/cm2 GFR-matrigel coated wells, in technical triplicates per line. Simvastatin-treated cells were exposed to 10 uM simvastatin for 48 hours. Sample collection was performed on ice. Conditioned culture medium was removed then cells were lifted using accutase and spun at 300 x g for 5 mins. Cell pellets were resuspended in PBS for counting and spun once more at 300 x g for 5 mins. Supernatants were aspirated and cell pellets were stored at -80 degrees C until sample processing.

Treatment ID:

TR003458

Treatment Summary:

iNSCs were seeded in NIM with Y-27632 (10 µM) at a density of 100,000 cells/cm2 GFR-matrigel coated wells, in technical triplicates per line. Simvastatin-treated cells were exposed to 10 uM simvastatin for 48 hours. Sample collection was performed on ice. Conditioned culture medium was removed then cells were lifted using accutase and spun at 300 x g for 5 mins. Cell pellets were resuspended in PBS for counting and spun once more at 300 x g for 5 mins. Supernatants were aspirated and cell pellets were stored at -80 degrees C until sample processing.