Summary of Study ST003492
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002141. The data can be accessed directly via it's Project DOI: 10.21228/M8VZ4T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003492 |
| Study Title | Metabolomic profiling of the secretome in normal primary human pulmonary artery smooth muscle cells |
| Study Summary | Growth medium of human pulmonary artery smooth muscle cells (PASMCs) was conditioned for 24 hours and analyzed by LC-MS, termed conditioned medium (CM). Comparing to fresh complete vascular smooth muscle cell growth medium (GM), LC-MS profiling screened 138 metabolites and identified that three branched chain alpha-ketoacids, namely KIC, KIV, and KMV are the most abundant metabolites in CM of human PASMCs. |
| Institute | Peking University |
| Department | Department of Toxicology |
| Last Name | Xiao |
| First Name | Wusheng |
| Address | 38 Xueyuan Rd, Haidian District, Beijing, Beijing, 100191, China |
| wxiao@bjmu.edu.cn | |
| Phone | +1-010-82801628 |
| Submit Date | 2023-08-07 |
| Analysis Type Detail | MALDI |
| Release Date | 2024-10-04 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR003629 |
| Treatment Summary: | Human primary pulmonary artery smooth muscle cells were cultured in complete vascular smooth muscle cell growth medium in a T75 flask. When cells reach to roungly 80% confluence, growth medium were refreshed with 10 mL and cultured for 24 hours. Medium samples were then collected (termed conditioned medium, CM) and used for LC-MS analysis after centrifugation and removal of cell debris. Regular complete growth medium (GM) of vascular smooth muscle cells was included for comparison. |
| Treatment: | Conditioned medium (CM) vs regular growth medium (GM) |
| Treatment Compound: | N/A |
| Treatment Route: | N/A |
| Treatment Dose: | N/A |
| Treatment Dosevolume: | N/A |
| Treatment Doseduration: | 24 hours |
| Treatment Vehicle: | N/A |
| Cell Growth Container: | 75 cm flasks |
| Cell Growth Config: | 1200 rpm |
| Cell Growth Rate: | 30-40 hours |
| Cell Media: | Complete vascular smooth muscle cell growth medium supplemented with growth factors and 5% FBS |
| Cell Envir Cond: | 37 C and 5% CO2 |
| Cell Pct Confluence: | 70-80% |
| Cell Media Lastchanged: | Every 48 hours |
