Summary of Study ST003608

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002231. The data can be accessed directly via it's Project DOI: 10.21228/M87R79 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003608
Study Title	Global metabolomics to compare metabolic profiles of live vs heat-killed thymidine-auxotrophe E. Coli
Study Summary	Global metabolomics of live and heat-killed thymidine-auxotrophe Escherichia Coli K12
Institute	University of Colorado Anschutz Medical Campus
Last Name	Haines
First Name	Julie
Address	12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA
Email	julie.haines@cuanschutz.edu
Phone	3037243339
Submit Date	2024-11-26
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2024-12-27
Release Version	1

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Treatment:

Treatment ID:	TR003746
Treatment Summary:	Preparation of viable and killed bacteria: ThyA- E. coli were grown overnight with shaking in LB supplemented with thymidine (500 ug/ml) and trimethoprim (50 ug/ml), diluted 1/40, and grown until log-phase [optical density at 600 nm (OD600) of 0.8-1.2]. Bacteria were washed with PBS and subjected to heat-killing by re-suspension in PBS and subsequently incubation at 60˚C for 60-90 min. Efficient killing was confirmed by overnight plating on LB-agar plates.

Treatment ID:

TR003746

Treatment Summary:

Preparation of viable and killed bacteria: ThyA- E. coli were grown overnight with shaking in LB supplemented with thymidine (500 ug/ml) and trimethoprim (50 ug/ml), diluted 1/40, and grown until log-phase [optical density at 600 nm (OD600) of 0.8-1.2]. Bacteria were washed with PBS and subjected to heat-killing by re-suspension in PBS and subsequently incubation at 60˚C for 60-90 min. Efficient killing was confirmed by overnight plating on LB-agar plates.