Summary of Study ST003643

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002253. The data can be accessed directly via it's Project DOI: 10.21228/M8DC25 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003643
Study TitleMetabolite analysis for WT and BnaMYB52 mutants by LC-MS/MS
Study SummaryBnaA09.MYB52 directly targets the BnaBAN promoters and promotes BnaBAN expression in Brassica napus. BAN, encoding anthocyanidin reductase that converts anthocyanidins to 2,3- cis-flavan-3-ols compounds (proanthocyanidins starter units), is involved in the flavonoid biosynthesis pathway. Thus, Metabolite analysis was conducted to detect the content of flavonoid in WT (Wild-type), OE (BnaA09.MYB52 overexpression lines in the genetic background Westar) and mutants (four homologous genes of BnaMYB52 knocked out) plants. About 0.1 g mature seeds were collected from WT, OE and mutant plants. Metabolites analysis demonstrated that BnaMYB52 positively regulated the content of several metabolites (such as L-phenylalanine, p-coumaric acid, grosvenorine and astragalin) in flavonoid pathway.
Institute
Huazhong Agricultural University
Last NameJiang
First NameYe
AddressShi Zishan Street 1th, Wuhan, Hubei, 430070, China
Emailvyejiang@163.com
Phone13697353446
Submit Date2024-12-21
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2025-01-26
Release Version1
Ye Jiang Ye Jiang
https://dx.doi.org/10.21228/M8DC25
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR003782
Treatment Summary:WT,OE and Homozygous mutants were grown in the genetically modified field of Huazhong Agricultural University in Wuhan from October 2022 to May 2023 and from October 2023 to May 2024. WT indicates Wide-type. OE indicates BnaA09.MYB52 overexpression lines in the genetic background Westar. Mutant indicates four homologous genes of BnaMYB52 knocked out.
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