Summary of Study ST003808

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002382. The data can be accessed directly via it's Project DOI: 10.21228/M8QZ7Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003808
Study TitleGlucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division
Study SummaryPeroxisomes play a crucial role in cellular metabolism. Glucose-6-phosphate dehydrogenase (G6PD), the gatekeeper enzyme of the pentose phosphate pathway, is primarily localized in the cytosol. However, studies have reported its presence in peroxisomes as well. This project aims to determine the function of G6PD on the peroxisomal membrane. In this study, we overexpressed G6PD in either the cytosol or on the peroxisomal membrane of mammary epithelial stem-like cells (hMECs). By comparing their lipidomic profiles, we found that peroxisomal membrane-associated G6PD provides NADPH, which feeds into peroxisomal ether lipid synthesis.
Institute
University of Helsinki
DepartmentFaculty of Biological and Environmental Sciences
LaboratoryKatajisto Laboratory
Last NameHien
First NameBui
AddressBiocenter 1, Viikinkaari 9
Emailhien.bui@helsinki.fi
Phone+358294159407
Submit Date2025-03-06
Raw Data AvailableYes
Raw Data File Type(s)cdf, mzdata.xml
Analysis Type DetailGC-MS/LC-MS
Release Date2025-03-24
Release Version1
Bui Hien Bui Hien
https://dx.doi.org/10.21228/M8QZ7Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR003951
Treatment Summary:Human mammary epithelial stem like cells (hMECs) were transfected with endotoxin-free G6PD overexpression plasmids using jetPRIME (Polyplus, 101000015). Cells were analyzed 24h post-transfection.
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