Summary of study ST000113

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000102. The data can be accessed directly via it's Project DOI: 10.21228/M88C7T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000113
Study TitleSIRM Analysis of human P493 cells under hypoxia in [U-13C/15N] labeled Glutamine medium (Positive ion mode FTMS)
Study Typetracer
Study SummarySIRM Analysis of MYC inducible P493 cells under normoxia and hypoxia in [U-13C/15N] labeled Glutamine medium
Institute
University of Kentucky
DepartmentToxicology
Last NameFan
First NameTeresa
Emailtwmfan@gmail.com
Submit Date2014-10-08
Num Groups4
Total Subjects8
Study CommentsFor protocols and additional information, see Le, A., Lane, A.N., Hamaker, M., Bose, S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. L.J., Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., and Dang, C.V. Cell Metabolism 15, 110 (2012). 13C/15N Gln was used as the tracer in this study, even though the analysis did not explicity look for 15N isotopologues.
Raw Data AvailableNo
Analysis Type DetailGC/LC-MS
Release Date2015-02-03
Release Version1
Teresa Fan Teresa Fan
https://dx.doi.org/10.21228/M88C7T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000102
Project DOI:doi: 10.21228/M88C7T
Project Title:SIRM Analysis of human P493
Project Summary:SIRM Analysis of human MYC-inducible P493 cells
Institute:Johns Hopkins University
Department:Pathology
Last Name:Dang
First Name:Chi
Email:cvdang@jhmi.edu

Subject:

Subject ID:SU000132
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:P493
Species Group:Human

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Oxygen Condition Treatment
SA006080hypox-13C/15N-Gln-plate-1hypoxia None
SA006081hypox-13C/15N-Gln-plate-2hypoxia None
SA006082tet-hypox-13C/15N-Gln-plate-1hypoxia Tetracycline
SA006083tet-hypox-13C/15N-Gln-plate-2hypoxia Tetracycline
SA006084normox-13C/15N-Gln-plate-2normoxia None
SA006085normox-13C/15N-Gln-plate-1normoxia None
SA006086tet-normox-13C/15N-Gln-plate-2normoxia Tetracycline
SA006087tet-normox-13C/15N-Gln-plate-1normoxia Tetracycline
Showing results 1 to 8 of 8

Collection:

Collection ID:CO000116
Collection Protocol Comments:media collection from plate | Collection and quench of cells in tissue culture
Sample Type:B Cells

Treatment:

Treatment ID:TR000134
Treatment Protocol Comments:tet-normox-13C/15N-Gln: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln | normox-13C/15N-Gln: cell culture under normoxia for 24hr + 24hr with [U-13C/15N]-Gln | hypox-13C/15N-Gln: cell culture under hypoxia for 24hr + 24hr with [U-13C/15N]-Gln | tet-hypox-13C/15N-Gln: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln

Sample Preparation:

Sampleprep ID:SP000129
Sampleprep Protocol Comments:Protein extraction and quantification. | Lipid extraction and preparation for FTMS. | Preparation of polar samples for GCMS and NMR.

Combined analysis:

Analysis ID AN000190 AN000191
Analysis type MS MS
Chromatography type GC GC
Chromatography system
Column
MS Type ESI EI
MS instrument type Single quadrupole GC-ITQ
MS instrument name Thermo LTQ-FT Thermo ITQ
Ion Mode POSITIVE POSITIVE
Units renormalized corrected Peak area normalized Peak area

Chromatography:

Chromatography ID:CH000127
Chromatography Type:GC

MS:

MS ID:MS000153
Analysis ID:AN000190
Instrument Name:Thermo LTQ-FT
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000154
Analysis ID:AN000191
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
Ion Mode:POSITIVE
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