Summary of study ST000136

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000121. The data can be accessed directly via it's Project DOI: 10.21228/M8XW20 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000136
Study TitleNIST lipidomics intralaboratory study
Study TypeDiet comparison
Study SummaryTargeted lipids were quantified and compared to the quantities of other labs across the nation. Comparisons were between pooled serum from humans who have consumed flax seed for the past month, pooled serums from humans who have consumed fish oil for the last month, and pooled serum from humans who have not consumed either flax seed or fish oil for the past month. Pooled samples were prepared in triplicate.
Institute
University of Florida
DepartmentCollege of Medicine, Department of Pathology, Immunology, and Laboratory Medicine
LaboratoryBiomedical Mass Spectometry Lab
Last NamePatterson
First NameRainey
Emailrpatterson@chem.ufl.edu
Submit Date2014-01-16
Num Groups4
Total Subjects12
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Rainey Patterson Rainey Patterson
https://dx.doi.org/10.21228/M8XW20
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000121
Project DOI:doi: 10.21228/M8XW20
Project Title:NIST lipidomics intralaboratory study
Project Type:Lipidomics, targeted
Project Summary:Relative quantities of lipids were compared between three different human serum sample sets and compared to the results of other laboratories. Human serum samples were pooled from people who had either ingested fish oil supplements, flax seed, or neither for the last month. NIST SRM 1950 human pooled blood plasma was used for quality control and method development. Inidvidual lipids among several classes were targeted, including phosphoglycerolipids, triacylglycerides, diacylglycerides, sphingolipids, cholesterol and cholesteryl esters.
Institute:University of Florida
Department:College of Medicine, Department of pathology, immunology, and laboratory medicine
Laboratory:Biomedical Mass Spectrometry Lab
Last Name:Garrett
First Name:Timothy
Email:tgarrett@ufl.edu
Funding Source:National Institutes of Health

Subject:

Subject ID:SU000155
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA007460SRM2378 1_Ccontrol
SA007461SRM2378 1_Acontrol
SA007462SRM2378 1_Bcontrol
SA007463SRM2378 3_Cfish oil
SA007464SRM2378 3_Afish oil
SA007465SRM2378 3_Bfish oil
SA007466SRM2378 2_Cflax seed
SA007467SRM2378 2_Aflax seed
SA007468SRM2378 2_Bflax seed
SA007457SRM 1950_AQC
SA007458SRM 1950_CQC
SA007459SRM 1950_BQC
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000139
Collection Protocol ID:SOP_LE_Matyash
Collection Protocol Filename:SOP_LE_02_Matyash_RP_NIST.pdf
Sample Type:blood
Storage Conditions:-80C

Treatment:

Treatment ID:TR000157

Sample Preparation:

Sampleprep ID:SP000152
Sampleprep Summary:MTBE lipid extraction
Sampleprep Protocol ID:SOP_LE_Matyash
Sampleprep Protocol Filename:SOP_LE_02_Matyash_RP_NIST.pdf
Processing Storage Conditions:On ice
Extraction Method:MTBE
Extract Concentration Dilution:40 uLplasma/40uL reconstitution solvent
Sample Resuspension:40 uL isopropanol
Sample Spiking:Internal standards listed in protocol
Organ:blodo plasma

Combined analysis:

Analysis ID AN000218
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000
Column Waters Acquity BEH C18 (50 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units nmol/mL serum

Chromatography:

Chromatography ID:CH000150
Chromatography Summary:Reverse phase
Methods Filename:m_lipids_AIF_pos
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (50 x 2.1mm, 1.7um)
Column Pressure:800 bar (max)
Column Temperature:35
Flow Gradient:none
Flow Rate:0.5 mL/min
Injection Temperature:4 C
Sample Injection:3 uL for pos mode, 10 uL for neg mode
Solvent A:60:40 acetonitrile:water 10 mM ammonium formate, 0.1% formic acid
Solvent B:90:8:2 isopropanol:acetonitrile:water, 10 mM ammonium formate, 0.1% formic acid
Analytical Time:20 min
Capillary Voltage:50 C
Running Voltage:3.5 kV
Randomization Order:3b,1b,1c,3c,2c,2a,2b,1a,3a
Chromatography Type:Reversed phase

MS:

MS ID:MS000170
Analysis ID:AN000218
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:300 (+), 250 (-)
Collision Energy:25 NCE
Dry Gas Flow:30 (+), 25 (-)
Ion Spray Voltage:3.5 kV
Mass Accuracy:< 2ppm
Precursor Type:Dependent upon class
Spray Voltage:3.5 kV
Dataformat:.RAW
Scan Range Moverz:120-2000
Skimmer Voltage:15
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