Summary of Study ST000188

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000159. The data can be accessed directly via it's Project DOI: 10.21228/M8P88W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000188
Study TitleMurine apyrase
Study SummaryThis experiment was performed in wild type vs. CD39 knockout (KO) mice that had received infusions of either saline or apyrase over the course of 2 weeks. Whole blood was obtained via intracardiac puncture, and was drawn into a syringe prefilled with an anti-enzyme stop solution.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-08
Num Groups4
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size32 M
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8P88W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000159
Project DOI:doi: 10.21228/M8P88W
Project Title:Murine AxP
Project Summary:Plasma Nucleotide/adenosine concentrations
Institute:University of Michigan
Department:Internal Medicine-Cardiology
Laboratory:Visovatti Lab
Last Name:Visovatti
First Name:Scott
Address:Ann Arbor, MI
Email:shv@med.umich.edu
Phone:734-657-6917

Subject:

Subject ID:SU000207
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Type Treatment
SA009614S00017521KO apyrase
SA009615S00017522KO apyrase
SA009616S00017523KO apyrase
SA009617S00017525KO apyrase
SA009618S00017524KO apyrase
SA009619S00017520KO saline
SA009620S00017519KO saline
SA009621S00017518KO saline
SA009622S00017517KO saline
SA009623S00017514wild type apyrase
SA009624S00017515wild type apyrase
SA009625S00017516wild type apyrase
SA009626S00017512wild type saline
SA009627S00017513wild type saline
SA009628S00017511wild type saline
Showing results 1 to 15 of 15

Collection:

Collection ID:CO000194
Collection Summary:-
Sample Type:Plasma

Treatment:

Treatment ID:TR000214

Sample Preparation:

Sampleprep ID:SP000208
Sampleprep Summary:-
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000289
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode NEGATIVE
Units nM

Chromatography:

Chromatography ID:CH000211
Methods ID:AQM016
Methods Filename:QQQ-ATP_ADP_AMP_A-update.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000238
Analysis ID:AN000289
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QQQ-ATP_ADP_AMP_A-update.m.zip
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