Summary of Study ST000199

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000154. The data can be accessed directly via it's Project DOI: 10.21228/M8688J This work is supported by NIH grant, U2C- DK119886.

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Study IDST000199
Study TitleIDH1 and Glioma knockdown idh1 (part II)
Study SummaryTumor neurospheres were grown in culture until 90% confluent. To collect cells, cells were filtered through 0.45um nylon filters (Fischer Sci 099029) that were prewashed with methanol and ultrapure water using a microanalytical glass filter holder (Fisher Sci 09753E). The cells were filtered through filtration apparatus, then washed with 1mL of distilled water very quickly. Filter paper is taken immediatedly with tweezers and plunged cell side down into 6-well plate filled with liquid nitrogen placed on dry ice. Plate is wrapped in aluminum foil and stored at -80C before the liquid nitrogen evaporates. Samples are shipped on dry ice
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-10
Num Groups2
Total Subjects10
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size16 M
Analysis Type DetailLC-MS
Release Date2015-12-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8688J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000154
Project DOI:doi: 10.21228/M8688J
Project Title:Isocitrate dehydrogenase-1 and Glioma Studies
Project Summary:Dr. Stegh will define the role of a novel glioma oncoprotein, termed isocitrate dehydrogenase-1 (IDH1), in driving progression and therapy resistance of glioblastoma (GBM). Understanding the molecular basis of the therapy refractoriness of GBM is one of the most important areas of glioma research.IDH1 is a critical enzyme of the citric acid cycle (CAC) and is a master regulator of metabolism. Building on his preliminary studies, Dr. Stegh will molecularly characterize the precise mechanism, by which IDH1 protects glioma cells from therapy-induced cell death using glioma cell and mouse models. To target IDH1 signaling in GBM, he will leverage these model systems and mechanistical knowledge to develop and preclinically characterize RNA interference RNAi-based nanomaterials. He will generate RNAi-functionalized spherical nucleic acids (SNAs) that neutralize IDH1 expression in established gliomas. Due to the negative charge of the RNA backbone, however, siRNA oligonucleotides have many downsides, such as they trigger auto-immune responses, and cannot cross the blood-brain-barrier (BBB). In contrast, SNAs are able to transverse cellular membranes, do not require the use of toxic auxiliary reagents, and accumulate in cells and intracranial tumors very effectively. They also exhibit extraordinary stability in physiological environments, cross the BBB, are highly resistant to nuclease degradation, and thus, can move through biological fluids and avoid being destroyed as “foreign materials.” Dr. Stegh proposes to preclinically evaluate these IDH1-targeting nanoconjugates to provide a fundamentally novel treatment option of patients diagnosed with GBM, and will aid in successfully implementing RNAi-based therapies into neuro-oncological practice.
Institute:Northwestern University
Department:Neurology
Laboratory:Stegh Lab
Last Name:Stegh
First Name:Alexander
Address:Evanston, IL
Email:a-stegh@northwestern.edu
Phone:312-503-2879

Subject:

Subject ID:SU000218
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Knockdown of IDH1
SA010025S00014999n/a
SA010026S00015002n/a
SA010027S00015003n/a
SA010028S00015001n/a
SA010029S00015000n/a
SA010030S00015007yes
SA010031S00015008yes
SA010032S00015005yes
SA010033S00015004yes
SA010034S00015006yes
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000206
Collection Summary:-
Sample Type:Cells, Cultured

Treatment:

Treatment ID:TR000226

Sample Preparation:

Sampleprep ID:SP000220
Sampleprep Summary:-
Sampleprep Protocol Filename:Acyl-carnitines_analysis_protocol-CR-MK-20150528.docx

Combined analysis:

Analysis ID AN000301
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1200
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units pmol/µg protein

Chromatography:

Chromatography ID:CH000222
Methods ID:AQM050
Methods Filename:QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip
Instrument Name:Agilent 1200
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000250
Analysis ID:AN000301
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Acquisition Parameters File:QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip
Processing Parameters File:EX00311-acyl-carnitines-quant.m.zip
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