Summary of Study ST000226

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000120. The data can be accessed directly via it's Project DOI: 10.21228/M82P4X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000226
Study TitleMetabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental Chemicals Induces Systemic Metabolic Dysfunction in Mice (Part I)
Study Typedrug dosage
Study Summary12 mice were acclimatized for a week, taught to eat dough pills then half were treated with TCDF per pill per day for 5 days Also 10 AHR -/- mice were used and treated the same way
Institute
Pennsylvania State University
DepartmentMolecular Toxicology
Last NamePatterson
First NameAndrew
Emailadp117@PSU.EDU
Submit Date2015-06-18
Raw Data AvailableNo
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2015-06-18
Release Version1
Andrew Patterson Andrew Patterson
https://dx.doi.org/10.21228/M82P4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000120
Project DOI:doi: 10.21228/M82P4X
Project Title:Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice
Institute:Pennsylvania State University
Department:Molecular Toxicology
Last Name:Patterson
First Name:Andrew
Email:adp117@PSU.EDU; RGN5011@psu.edu

Subject:

Subject ID:SU000245
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J; Wild Type and Ahr-/-
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Gender Genotype Treatment
SA010810Treated serum_Ahr-Male Ahr -/- TCDF
SA010806Treated LiverMale Ahr +/+ TCDF
SA010807Treated serum_Ahr+Male Ahr +/+ TCDF
SA010811Control serum_Ahr-Male Ahr -/- Vehicle
SA010808Control LiverMale Ahr +/+ Vehicle
SA010809Control serum_Ahr+Male Ahr +/+ Vehicle
Showing results 1 to 6 of 6

Collection:

Collection ID:CO000233
Collection Summary:-
Sample Type:Feces, liver, cecal contents, intestine and blood (only liver and blood tested with NRM)

Treatment:

Treatment ID:TR000253
Treatment Summary:Wild type and AHR knock out mice (12 wild types and 10 AHR knock out) were treated with TCDF for 5 days
Treatment:Dietary
Treatment Compound:TCDF
Treatment Route:Oral
Treatment Dose:24 µg/kg body weight for AHR and 5 µg/kg body weight for wild type

Sample Preparation:

Sampleprep ID:SP000247
Sampleprep Summary:-
Sampleprep Protocol Filename:NMR_metabolomics_protocol.docx
Processing Method:Homogenization
Processing Storage Conditions:-20
Extraction Method:Methanol/Water
Extract Concentration Dilution:2 Methanol : 1 Water
Sample Resuspension:D2O
Sample Spiking:TSP-d4
Organ:Liver, small and large intestine

Analysis:

Analysis ID:AN000336
Analysis Type:NMR
Chromatography ID:CH000252
Num Factors:4

NMR:

NMR ID:NM000055
Analysis ID:AN000336
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:NOESY PR1D
Spectrometer Frequency:600 MHz
NMR Solvent:D2O
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