Summary of Study ST000249
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000201. The data can be accessed directly via it's Project DOI: 10.21228/M8DK5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000249 |
| Study Title | Measurement of free amino acid (AA) in response to MYC |
| Study Type | treatment |
| Study Summary | The proposed study will provide us information how the different AAs are affected by MYC. MYC is known to cause increased levels of glutamin due to import and synthesis, but we hypothesize that the overall pool of free AA in the cells is reduced, especially when cells are starved. |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Schaub |
| First Name | Franz |
| Franz.Schaub@moffitt.org | |
| Submit Date | 2015-09-22 |
| Num Groups | 2 |
| Total Subjects | 6 |
| Raw Data Available | No |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000201 |
| Project DOI: | doi: 10.21228/M8DK5C |
| Project Title: | Myc-directed suppression of autophagy provides therapeutic vulnerabilities targeting amino acid homeostasis |
| Project Type: | Targeted LCMS Metabolomics |
| Project Summary: | Hypothesis:MYC is suppressing the autophagy pathway which leads to impaired recycling of proteins and reduced free AA in the cells. |
| Institute: | Moffitt Cancer Center |
| Department: | Tumor Biology |
| Laboratory: | John Cleveland |
| Last Name: | Cleveland |
| First Name: | John |
| Address: | 12902 Magnolia Dr,Tampa, FL 33612 |
| Phone: | 813 745 3888 |
| Funding Source: | NIH |
Subject:
| Subject ID: | SU000269 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA011384 | 48hr_TET_2 | 48 hour Tet treatment |
| SA011385 | 48hr_TET_3 | 48 hour Tet treatment |
| SA011386 | 48hr_TET_1 | 48 hour Tet treatment |
| SA011387 | 48hr_NoTET_3 | No Tet treatment |
| SA011388 | 48hr_NoTET_2 | No Tet treatment |
| SA011389 | 48hr_NoTET_1 | No Tet treatment |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO000260 |
| Collection Summary: | Cells were counted, washed with cold PBS and then flash-frozen in liquid N2 |
| Sample Type: | Cells |
| Volumeoramount Collected: | 1.00E7 |
Treatment:
| Treatment ID: | TR000280 |
| Treatment Summary: | Cells were treated with TET for 48 hrs (TET samples) and then washed and cultured without TET for 48hrs (noTET samples) |
Sample Preparation:
| Sampleprep ID: | SP000274 |
| Sampleprep Summary: | Amino acid |
| Sampleprep Protocol Filename: | SB_AA_Assay.pdf |
Combined analysis:
| Analysis ID | AN000393 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | nmol/million cells |
Chromatography:
| Chromatography ID: | CH000279 |
| Chromatography Summary: | Amino Acid |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000337 |
| Analysis ID: | AN000393 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
