Summary of study ST000260

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000211. The data can be accessed directly via it's Project DOI: 10.21228/M84598 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000260
Study TitleAnalysis of DJ-1 Knockout Mouse Brains
Study TypeMouse genotype comparison
Study SummaryWe analyzed metabolites in the brains of wild type mice and DJ-1 knockout mice. The DJ-1 knockout mouse is a model of an inherited form of Parkinson's disease.
Institute
National Institute on Aging
DepartmentLaboratory of Neurogenetics
LaboratoryCell Biology and Gene Expression Section
Last NameHauser
First NameDavid
Address35 Lincoln Drive, BLDG 35, Room 1A-1012, Bethesda, MD 20892
Emailhauserd@mail.nih.gov
Phone301-435-8995
Submit Date2015-09-17
Num Groups2
Total Subjects25
Raw Data AvailableNo
Analysis Type DetailGC/LC-MS
Release Date2017-10-03
Release Version1
David Hauser David Hauser
https://dx.doi.org/10.21228/M84598
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000211
Project DOI:doi: 10.21228/M84598
Project Title:Metabolic Response to the Loss of DJ-1
Project Type:Comparison of the metabolic responses to the loss of DJ-1 in model organisms
Project Summary:Mutations in the DJ-1 gene cause rare forms of autosomal recessive Parkinson's disease in humans, and increased levels of DJ-1 protein are often found in cancers. Thus, understanding the precise function of DJ-1 is of broad interest. We hypothesized that analyzing differences in metabolites between organisms with and without DJ-1 may lead to clues about the protein's function. Here, we present the results of our ongoing studies using multiple different model organisms.
Institute:National Institute on Aging
Department:Laboratory of Neurogenetics
Laboratory:Cell Biology and Gene Expression Section
Last Name:Cookson
First Name:Mark
Address:35 Lincoln Drive, BLDG 35, Room 1A116, Bethesda, MD, 20892
Email:cookson@mail.nih.gov
Funding Source:The NIH Intramural Program, National Institute on Aging

Subject:

Subject ID:SU000280
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57Bl6
Age Or Age Range:4 Months
Gender:Mixed
Animal Feed:Ad Libitum
Animal Water:Ad Libitum
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sex Genotype
SA0119701020F DJ-1 Knockout
SA0119711022F DJ-1 Knockout
SA011972836F DJ-1 Knockout
SA011973517F DJ-1 Knockout
SA011974521F DJ-1 Knockout
SA011975520F DJ-1 Knockout
SA011976823F WT
SA011977825F WT
SA011978518F WT
SA011979516F WT
SA011980560F WT
SA011981519M DJ-1 Knockout
SA011982828M DJ-1 Knockout
SA0119831030M DJ-1 Knockout
SA0119841035M DJ-1 Knockout
SA011985829M DJ-1 Knockout
SA011986563M DJ-1 Knockout
SA0119871033M WT
SA011988565M WT
SA011989564M WT
SA011990567M WT
SA0119911019M WT
SA0119921032M WT
SA0119931031M WT
SA011994515M WT
Showing results 1 to 25 of 25

Collection:

Collection ID:CO000274
Sample Type:Brain - Cerebral Hemisphere

Treatment:

Treatment ID:TR000294
Treatment Summary:The animals were either wild type of homozygous DJ-1 knockout animals. At 4 months of age, the mice were euthanized and their brains were processed for metabolomic analysis.
Animal Vet Treatments:None
Animal Fasting:No dietary interventions
Animal Endp Euthanasia:The mice were euthanized with CO2 followed by cervical dislocation.
Animal Endp Tissue Coll List:Cerebral Hemispheres
Animal Endp Tissue Proc Method:Immediately after euthanization, the brains were removed and dissected on an ice cold stage. The cerebellum was removed and the two cerebral hemispheres were bisected along the longtiduinal fissue. The cerebral hemispheres were immediately frozen on dry ice in individual tubes. The frozen tissue was then sent to Metabolon for subsequent processing.

Sample Preparation:

Sampleprep ID:SP000288
Sampleprep Summary:Frozen cerebral hemispheres were shipped to Metabolon and processed there. A detailed summary of their methods is unavalible.

Combined analysis:

Analysis ID AN000413 AN000414 AN000415
Analysis type MS MS MS
Chromatography type GC GC GC
Chromatography system
Column
MS Type ESI ESI EI
MS instrument type Single quadrupole Single quadrupole Single quadrupole
MS instrument name Thermo LTQ Thermo LTQ Thermo Trace DSQ
Ion Mode POSITIVE NEGATIVE POSITIVE
Units Area Area

Chromatography:

Chromatography ID:CH000292
Chromatography Type:GC

MS:

MS ID:MS000355
Analysis ID:AN000413
Instrument Name:Thermo LTQ
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000356
Analysis ID:AN000414
Instrument Name:Thermo LTQ
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000357
Analysis ID:AN000415
Instrument Name:Thermo Trace DSQ
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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