Summary of study ST000301

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000242. The data can be accessed directly via it's Project DOI: 10.21228/M8CG6D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download all metabolite data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data (Contains raw data)
Study IDST000301
Study TitleGBM Cell Lines Reproducibility Pilot Study
Study SummaryGBM cell lines were plated onto 10 cm dishes at 100,000-200,000 cells per dish and allowed to grow for 3-4 days until confluency reached 50-70%. Media for all cell lines was DMEM with 10% FBS including 25 mM glucose, 6.2 mM glutamine and 200 uM Oleic Acid (conjugated to BSA). 1-2 hours prior to tracer incubation, media was aspirated and fresh media was used. Immediately prior to tracer incubation, media was again aspirated and then cells were washed with warm PBS to remove unlabeled metabolites. Cells were then incubated with labeled tracer (DMEM with 10% FBS including 25 mM Uniformly labeled 13C-6 glucose, 6.2 mM unlabeled glutamine and 200 uM unlabeled oleic acid). After 2 hours of incubation with tracer, media was aspirated, cells were quickly washed with 15 mL DI water, quenched with liquid nitrogen and then stored at -80oC until analysis.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-03-17
Num Groups10
Total Subjects28
Raw Data AvailableYes
Raw Data File Type(s).xml, .bin, .xsd
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8CG6D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000242
Project DOI:doi: 10.21228/M8CG6D
Project Title:DRW GBM Cell Line Metabolomics
Project Summary:Profiling 13C glucose flux-based metabolome across GBM cell lines to determine metabolic predictors of radiation sensitivity
Institute:University of Michigan
Department:Radiation Oncology
Last Name:Lawrence
First Name:Theodore
Address:Ann Arbor, MI
Email:tsl@umich.edu
Phone:734-647-9955

Subject:

Subject ID:SU000321
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell Line
SA013608S00017760L229
SA013609S00017768L229
SA013610S00017770L229
SA013611S00017759L229
SA013612S00017769L229
SA013613S00017761L229
SA013614S00017752L229
SA013615S00017750L229
SA013616S00017751L229
SA013617S00017754LN18
SA013618S00017771LN18
SA013619S00017773LN18
SA013620S00017772LN18
SA013621S00017763LN18
SA013622S00017764LN18
SA013623S00017762LN18
SA013624S00017753LN18
SA013625S00017755LN18
SA013626S00020346Pooled glioblastoma
SA013627S00017748U-138
SA013628S00017756U-138
SA013629S00017757U-138
SA013630S00017758U-138
SA013631S00017747U-138
SA013632S00017765U-138
SA013633S00017766U-138
SA013634S00017767U-138
SA013635S00017749U-138
Showing results 1 to 28 of 28

Collection:

Collection ID:CO000315
Collection Summary:-
Sample Type:Glioblastoma cells

Treatment:

Treatment ID:TR000335
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000329
Sampleprep Summary:-
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.docx

Combined analysis:

Analysis ID AN000479
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520 QTOF
Ion Mode NEGATIVE
Units Area

Chromatography:

Chromatography ID:CH000342
Methods Filename:QTOF-002-HILIC-35min-1mm_bottompheater-no_insert.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS000419
Analysis ID:AN000479
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  logo