Summary of Study ST000374

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000295. The data can be accessed directly via it's Project DOI: 10.21228/M8X60B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000374
Study TitleMice exercise metabolomics (part I)
Study SummaryMice were run for 15 minutes on a treadmill and then mice were induced into anesthesia at a dose of 5% isoflurane, then maintained by continuous inhalation of 2% isoflurane. Quadriceps muscle was collected first and flash frozen in liquid nitrogen. Then blood was collected from the left ventricle of the heart and placed into serum separating tubes. Blood was allowed to clot for 40 minutes and centrifuged, the supernatant was frozen in liquid nitrogen
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryFacilities Metabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-04-21
Num Groups4
Total Subjects18
Study CommentsWTvs113Q
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8X60B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000295
Project DOI:doi: 10.21228/M8X60B
Project Title:Metabolomics Mice Studies
Project Summary:Analysis of glycolysis/TCA cycle in skeletal muscle + acyl-carnitine in serum of wt and 113Q mice
Institute:University of Michigan
Department:Pathology
Laboratory:Lieberman, Andrew
Last Name:Lieberman
First Name:Andrew
Address:Ann Arbor, MI
Email:liebermn@umich.edu
Phone:734-936-1889

Subject:

Subject ID:SU000395
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Wild Type Mutants
SA016986S00018468no yes
SA016987S00018474no yes
SA016988S00018473no yes
SA016989S00018467no yes
SA016990S00018465no yes
SA016991S00018475no yes
SA016992S00018466no yes
SA016993S00018476no yes
SA016994S00018472yes no
SA016995S00018469yes no
SA016996S00018463yes no
SA016997S00018462yes no
SA016998S00018464yes no
SA016999S00018461yes no
SA017000S00018470yes no
SA017001S00018471yes no
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000389
Collection Summary:none
Sample Type:Serum

Treatment:

Treatment ID:TR000409
Treatment Summary:none

Sample Preparation:

Sampleprep ID:SP000402
Sampleprep Summary:none
Sampleprep Protocol Filename:Acyl-carnitines analysis protocol-CR-MK-20150528.docx

Combined analysis:

Analysis ID AN000608
Analysis type MS
Chromatography type Reversed phase
Chromatography system Unspecified
Column Waters ACQUITY UPLC RP18 (50 x 2.1mm)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode POSITIVE
Units ┬ÁM

Chromatography:

Chromatography ID:CH000434
Chromatography Summary:none
Methods ID:DQM0138
Methods Filename:QM-004-Xbridg2mm_ACar+_MRM-Insert-gradtest3.m.zip
Instrument Name:Unspecified
Column Name:Waters ACQUITY UPLC RP18 (50 x 2.1mm)
Chromatography Type:Reversed phase

MS:

MS ID:MS000541
Analysis ID:AN000608
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:QM-004-Xbridg2mm_ACar+_MRM-Insert-gradtest3.m.zip
  logo