Summary of Study ST000377

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000296. The data can be accessed directly via it's Project DOI: 10.21228/M8SC7G This work is supported by NIH grant, U2C- DK119886.

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Study IDST000377
Study TitleKidney choline acetyltransferase (ChAT)-(part II)
Study TypeRegular
Study SummaryRats were treated with donepezil (DPZ) after the induction of a model of glomerulonephritis (GN) to increase kidney ACh levels. Rats were euthanized at day 25 after the induction of the disease. Kidneys were rapidly removed, frozen in liquid nitrogen and stored at -80C
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-04-16
Num Groups4
Total Subjects19
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8SC7G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000296
Project DOI:doi: 10.21228/M8SC7G
Project Title:Role of kidney acetylcholine in protection from kidney injury
Project Summary:ChAT was induced in a model of kidney inflammation to determine its role in kidney protection
Institute:University of Colorado Denver
Department:Medicine
Laboratory:Garcia Lab
Last Name:Garcia
First Name:Gabriela
Address:Denver, CO
Email:Gabriela.garcia@ucdenver.edu
Phone:303-724-4897

Subject:

Subject ID:SU000398
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Disease Treated
SA017043S00018118GN Treated
SA017044S00018110GN Treated
SA017045S00018121GN Treated
SA017046S00018117GN Treated
SA017047S00018120GN Treated
SA017048S00018119GN Treated
SA017049S00018116GN Treated
SA017050S00018111GN Treated
SA017051S00018112GN Treated
SA017052S00018124GN Vehicle
SA017053S00018123GN Vehicle
SA017054S00018122GN Vehicle
SA017055S00018115GN Vehicle
SA017056S00018113GN Vehicle
SA017057S00018114GN Vehicle
SA017058S00018127Nl Control
SA017059S00018125Nl Control
SA017060S00018126Nl Control
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000392
Collection Summary:none
Sample Type:Pooled sample

Treatment:

Treatment ID:TR000412
Treatment Summary:none

Sample Preparation:

Sampleprep ID:SP000405
Sampleprep Summary:none
Sampleprep Protocol Filename:2014-10-07-Neurotransmitter.docx

Combined analysis:

Analysis ID AN000611
Analysis type MS
Chromatography type Normal phase
Chromatography system None
Column Cogent Diamond Hydride
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode POSITIVE
Units pmol/mg sample

Chromatography:

Chromatography ID:CH000437
Methods ID:DQM0150
Methods Filename:QM009_2mm_DH_Neurotransmitter_MRM-ESP-1.m.zip
Instrument Name:None
Column Name:Cogent Diamond Hydride
Chromatography Type:Normal phase

MS:

MS ID:MS000544
Analysis ID:AN000611
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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