Summary of Study ST000420

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000329. The data can be accessed directly via it's Project DOI: 10.21228/M8R88H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)
Study IDST000420
Study TitleNIST SRM Smoker non-Smoker Urine Comparison
Study SummaryThis study is a test of the untargeted metabolomics approach to studying samples from exposure studies. The samples are NIST standard SRM 3672 for pooled urine of smokers and SRM 3673 for pooled urine of non-smokers. A broad spectrum GCMS approach was used in this study.
Institute
RTI International
DepartmentDiscovery Sciences
Last NameBurgess
First NameJason
Address3040 Cornwallis Rd, RTP, NC, 27609, USA
Emailjpb@rti.org
Phone919-541-6700
Submit Date2016-06-10
Study CommentsCHEAR ring trial profiiency testing
Chear StudyYes
Analysis Type DetailGC-MS
Release Date2024-12-31
Release Version1
Jason Burgess Jason Burgess
https://dx.doi.org/10.21228/M8R88H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000329
Project DOI:doi: 10.21228/M8R88H
Project Title:NIST SRM Smoker non-Smoker Urine Comparison
Project Type:Comparison
Project Summary:This study is a test of the untargeted metabolomics approach to studying samples from exposure studies. The samples are NIST standard SRM 3672 for pooled urine of smokers and SRM 3673 for pooled urine of non-smokers. A broad spectrum GCMS approach was used in this study.
Institute:RTI International
Department:Discovery Sciences
Last Name:Burgess
First Name:Jason
Address:3040 Cornwallis Rd, RTP, NC, 27609, USA
Email:jpb@rti.org
Phone:919-541-6700
Funding Source:NIEHS

Subject:

Subject ID:SU000441
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA021115N3673-05nonsmoker
SA021116N3673-04nonsmoker
SA021117N3673-03nonsmoker
SA021118N3673-06nonsmoker
SA021119N3673-08nonsmoker
SA021120N3673-10nonsmoker
SA021121N3673-09nonsmoker
SA021122N3673-02nonsmoker
SA021123N3673-07nonsmoker
SA021124N3673-01nonsmoker
SA021125N3672-05smoker
SA021126N3672-04smoker
SA021127N3672-03smoker
SA021128N3672-02smoker
SA021129N3672-06smoker
SA021130N3672-07smoker
SA021131N3672-10smoker
SA021132N3672-09smoker
SA021133N3672-08smoker
SA021134N3672-01smoker
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000435
Collection Summary:Typical Urine Collection Procedure
Sample Type:Urine

Treatment:

Treatment ID:TR000455
Treatment Summary:none

Sample Preparation:

Sampleprep ID:SP000448
Sampleprep Summary:Aliquots of 200 µL human urine were mixed with 20uL of urease suspension placed on Thermomixer for 1 hour at 37°C on 1400rpm. Then added 1.7mL of methanol, vortexed for 5 minutes, centrifuged for 10 minutes on 14,000 rpm at 4°C. 1.5 mL of the supernatant was transferred to new individual 2 mL Eppendorf tubes and dried completely under nitrogen flow. Placed on vacuum centrifuge for 30 min to ensure complete dryness. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Combined analysis:

Analysis ID AN000662
Analysis type MS
Chromatography type GC
Chromatography system Agilent
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units intensity

Chromatography:

Chromatography ID:CH000479
Instrument Name:Agilent
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Flow Gradient:constant
Flow Rate:1ml/min
Injection Temperature:250C
Retention Index:FAMES retention index C8-C30
Chromatography Type:GC

MS:

MS ID:MS000588
Analysis ID:AN000662
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
Ion Mode:POSITIVE
  logo