Summary of Study ST000420
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000329. The data can be accessed directly via it's Project DOI: 10.21228/M8R88H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000420 |
Study Title | NIST SRM Smoker non-Smoker Urine Comparison |
Study Summary | This study is a test of the untargeted metabolomics approach to studying samples from exposure studies. The samples are NIST standard SRM 3672 for pooled urine of smokers and SRM 3673 for pooled urine of non-smokers. A broad spectrum GCMS approach was used in this study. |
Institute | RTI International |
Department | Discovery Sciences |
Last Name | Burgess |
First Name | Jason |
Address | 3040 Cornwallis Rd, RTP, NC, 27609, USA |
jpb@rti.org | |
Phone | 919-541-6700 |
Submit Date | 2016-06-10 |
Study Comments | CHEAR ring trial profiiency testing |
Chear Study | Yes |
Analysis Type Detail | GC-MS |
Release Date | 2024-12-31 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000329 |
Project DOI: | doi: 10.21228/M8R88H |
Project Title: | NIST SRM Smoker non-Smoker Urine Comparison |
Project Type: | Comparison |
Project Summary: | This study is a test of the untargeted metabolomics approach to studying samples from exposure studies. The samples are NIST standard SRM 3672 for pooled urine of smokers and SRM 3673 for pooled urine of non-smokers. A broad spectrum GCMS approach was used in this study. |
Institute: | RTI International |
Department: | Discovery Sciences |
Last Name: | Burgess |
First Name: | Jason |
Address: | 3040 Cornwallis Rd, RTP, NC, 27609, USA |
Email: | jpb@rti.org |
Phone: | 919-541-6700 |
Funding Source: | NIEHS |
Subject:
Subject ID: | SU000441 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Group |
---|---|---|
SA021115 | N3673-05 | nonsmoker |
SA021116 | N3673-04 | nonsmoker |
SA021117 | N3673-03 | nonsmoker |
SA021118 | N3673-06 | nonsmoker |
SA021119 | N3673-08 | nonsmoker |
SA021120 | N3673-10 | nonsmoker |
SA021121 | N3673-09 | nonsmoker |
SA021122 | N3673-02 | nonsmoker |
SA021123 | N3673-07 | nonsmoker |
SA021124 | N3673-01 | nonsmoker |
SA021125 | N3672-05 | smoker |
SA021126 | N3672-04 | smoker |
SA021127 | N3672-03 | smoker |
SA021128 | N3672-02 | smoker |
SA021129 | N3672-06 | smoker |
SA021130 | N3672-07 | smoker |
SA021131 | N3672-10 | smoker |
SA021132 | N3672-09 | smoker |
SA021133 | N3672-08 | smoker |
SA021134 | N3672-01 | smoker |
Showing results 1 to 20 of 20 |
Collection:
Collection ID: | CO000435 |
Collection Summary: | Typical Urine Collection Procedure |
Sample Type: | Urine |
Treatment:
Treatment ID: | TR000455 |
Treatment Summary: | none |
Sample Preparation:
Sampleprep ID: | SP000448 |
Sampleprep Summary: | Aliquots of 200 µL human urine were mixed with 20uL of urease suspension placed on Thermomixer for 1 hour at 37°C on 1400rpm. Then added 1.7mL of methanol, vortexed for 5 minutes, centrifuged for 10 minutes on 14,000 rpm at 4°C. 1.5 mL of the supernatant was transferred to new individual 2 mL Eppendorf tubes and dried completely under nitrogen flow. Placed on vacuum centrifuge for 30 min to ensure complete dryness. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
Combined analysis:
Analysis ID | AN000662 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC x GC-TOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF |
Ion Mode | POSITIVE |
Units | intensity |
Chromatography:
Chromatography ID: | CH000479 |
Instrument Name: | Agilent |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Flow Gradient: | constant |
Flow Rate: | 1ml/min |
Injection Temperature: | 250C |
Retention Index: | FAMES retention index C8-C30 |
Chromatography Type: | GC |
MS:
MS ID: | MS000588 |
Analysis ID: | AN000662 |
Instrument Name: | Leco Pegasus 4D GCxGC TOF |
Instrument Type: | GC x GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |