Summary of study ST000445

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000343. The data can be accessed directly via it's Project DOI: 10.21228/M8JS49 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000445
Study TitleFollicular fluid lipidomics reveals lipid alterations by LH addition during IVF cycles
Study SummaryPurpose Ovulation induction protocols are key components for performing assisted reproduction treatments successfully. The objective of the present study was to estimate how LH addition to controlled ovarian stimulation protocols may affect the follicular fluid lipid profile of women undergoing in vitro fertilization treatment. Methods We conducted the study using 28 self-paired samples, 14 per group. The patients received FSH during their first cycle of ovarian stimulation (FSH group). If treatment did not result in pregnancy, the same patients returned for a new cycle and received stimulus with the addition of LH to the previous protocol (Low-dose-LH group). Lipidomics analysis was performed by UPLC-MSE mass spectrometry. Potential lipid biomarkers were identified by the software Progenesis QI. Statistical analysis was performed using the SPSS 18.0 and MetaboAnalyst 2.0 software.
Institute
Universidade Federal de Sao Paulo
DepartmentSurgery
LaboratoryCentro de Pesquisa em Urologia
Last NameDa Costa
First NameLivia
AddressRua Embau 231 - Vila Clementino, Sao Paulo, Sao Paulo, 04039060, Brazil
Emailliviadovale@hotmail.com
Phone551138074062
Submit Date2016-07-25
Num Groups2
Total Subjects28
Num Females14
Study CommentsThe groups consists of the same 14 women submitted to two different controlled ovarian stimulation protocols (FSH or LH group)
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Livia Da Costa Livia Da Costa
https://dx.doi.org/10.21228/M8JS49
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000343
Project DOI:doi: 10.21228/M8JS49
Project Title:Follicular fluid lipidomics reveals lipid alterations by LH addition during IVF cycles
Project Summary:Ovulation induction protocols are key components for performing assisted reproduction treatments successfully. The objective of the present study was to estimate how LH addition to controlled ovarian stimulation protocols may affect the follicular fluid lipid profile of women undergoing in vitro fertilization treatment. We conducted the study using 28 self-paired samples, 14 per group. The patients received FSH during their first cycle of ovarian stimulation (FSH group). If treatment did not result in pregnancy, the same patients returned for a new cycle and received stimulus with the addition of LH to the previous protocol (Low-dose-LH group). Lipidomics analysis was performed by UPLC-MSE mass spectrometry. Potential lipid biomarkers were identified by the software Progenesis QI. Statistical analysis was performed using the SPSS 18.0 and MetaboAnalyst 2.0 software.
Institute:Universidade Federal de Sao Paulo
Department:Surgery
Laboratory:Centro de Pesquisa em Urologia
Last Name:da Costa
First Name:Livia
Address:Rua Embau 231 - Vila Clementino, Sao Paulo, Sao Paulo, 04039060, Brazil
Email:liviadovale@hotmail.com
Phone:551138074062

Subject:

Subject ID:SU000466
Subject Type:Human patients (Women)
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:<= 37 years
Gender:Female
Species Group:Human

Factors:

Subject type: Human patients (Women); Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA022624UNIFESP_lip_LF12FSH
SA022625UNIFESP_lip_LF10FSH
SA022626UNIFESP_lip_LF13FSH
SA022627UNIFESP_lip_LF14FSH
SA022628UNIFESP_lip_LF1FSH
SA022629UNIFESP_lip_LF9FSH
SA022630UNIFESP_lip_LF11FSH
SA022631UNIFESP_lip_LF4FSH
SA022632UNIFESP_lip_LF8FSH
SA022633UNIFESP_lip_LF2FSH
SA022634UNIFESP_lip_LF5FSH
SA022635UNIFESP_lip_LF3FSH
SA022636UNIFESP_lip_LF6FSH
SA022637UNIFESP_lip_LF7FSH
SA022638UNIFESP_lip_LF25LH
SA022639UNIFESP_lip_LF24LH
SA022640UNIFESP_lip_LF26LH
SA022641UNIFESP_lip_LF28LH
SA022642UNIFESP_lip_LF23LH
SA022643UNIFESP_lip_LF27LH
SA022644UNIFESP_lip_LF15LH
SA022645UNIFESP_lip_LF17LH
SA022646UNIFESP_lip_LF16LH
SA022647UNIFESP_lip_LF18LH
SA022648UNIFESP_lip_LF19LH
SA022649UNIFESP_lip_LF21LH
SA022650UNIFESP_lip_LF20LH
SA022651UNIFESP_lip_LF22LH
Showing results 1 to 28 of 28

Collection:

Collection ID:CO000460
Collection Summary:The remaining follicular fluid from follicles aspiration (approximately 15 mL/patient) was centrifuged at 800 x g for 10 minutes to remove residual cells, and the supernatant was collected and stored at -20 °C until lipid analysis.
Sample Type:Follicular Fluid
Collection Method:Ovarian pucture
Collection Location:Ovarian follicle
Collection Frequency:One time

Treatment:

Treatment ID:TR000480
Treatment Summary:To induce follicular growth, each of the eligible 14 patients received both rFSH 225 IU alone (Gonal F® [Merck-Serono, Darmstadt, Germany]) (FSH group) and rFSH 225 IU + hMG 75 IU (Menopur® [Ferring, Kiel, Germany]) (Low-dose-LH group) starting on cycle day 2 in two separate cycles (i.e. each patient acted as her own control). Sequential transvaginal ultrasounds were performed from stimulation day 5 onwards. Daily doses of GnRH antagonist analog cetrorelix acetate 0.25 mg (Cetrotide® [Merck-Serono, Darmstadt, Germany]) were administered in both groups when the leading follicle reached a mean diameter of 13 mm until the stimulation day 7, or from day 8. When at least one follicle was greater than 17 mm, 250 mcg of human chorionic gonadotropin (hCG) (Ovidrel® [Merck-Serono, Darmstadt, Germany]) was administered, and oocyte retrieval was scheduled 35-36 hours later.
Treatment:Two types of controlled ovarian stimulation
Treatment Compound:Exogenous gonadotropins to induce follicular growth

Sample Preparation:

Sampleprep ID:SP000473
Sampleprep Summary:After thawing, lipids and metabolites were extracted based on the Bligh and Dyer protocol [26]. Briefly, 50 µL of sample was placed in a microtube, and 125 μL of chloroform (CHCl3) (Merck-Serono, Darmstadt, Germany) and 250 μL of methanol (CH3OH) (Merck-Serono, Darmstadt, Germany) were added. The mixture was submitted to homogenization for 2 minutes. The polar and nonpolar phases were separated by the addition of 125 μL of CHCl3 and 100 μL of Milli-Q water. Then, the samples were centrifuged for 5 minutes at 1000 x g. The organic phase (bottom) containing the lipids was recovered and transferred to a clean microtube, which was left open overnight at room temperature until the complete solvent evaporation.
Extraction Method:Modified Bligh and Dyer protocol

Combined analysis:

Analysis ID AN000696
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity I-Class
Column Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Synapt G2 S QTOF
Ion Mode POSITIVE
Units m/z intensity

Chromatography:

Chromatography ID:CH000504
Chromatography Summary:Reversed-phase analysis was performed on a Waters Ultra Performance liquid chromatography Acquity I-Class system using an Acquity HSS T3 2.10 x 100 mm column (Waters, Milford, USA). The column was maintained at 55 °C. The lipid extracts were analyzed by UPLC-MSE by injecting 10 μl of each sample dissolved in 1 mL of water:acetonitrile:2-propanol (H2O:ACN:2-propanol; 1:1:2, v:v:v) (Merck, Darmstadt, Germany). The chromatographic run was carried out in gradient mode with a flow rate of 0.5 mL min-1 with initial composition of 50% solvent B (ACN:2-propanol; 10:90) in solvent A (H2O:ACN; 40:60, v:v + 10 mM ammonium acetate (NH4Ac) (Sigma-Aldrich, Saint Louis, USA), maintained for 0 to 0.5 min. The gradient composition was changed linearly from 50% to 100% solvent B between 0.5 to 4.5 minutes, returning to the initial composition of 50% B in 4.6 minutes, kept to 5.0 minutes.
Instrument Name:Waters Acquity I-Class
Column Name:Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
Column Temperature:55ºC
Flow Rate:0.5 mL.min-1
Solvent A:H2O:ACN; 40:60, v:v + 10 mM ammonium acetate
Solvent B:ACN:2-propanol; 10:90
Chromatography Type:Reversed phase

MS:

MS ID:MS000618
Analysis ID:AN000696
Instrument Name:Waters Synapt G2 S QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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