Summary of Study ST000480

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000365. The data can be accessed directly via it's Project DOI: 10.21228/M8VW2C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000480
Study TitleNMR based quantitative metabolomics studies of heart and lung from mouse exposed to whole body high dose gamma and proton radiation (part II)
Study SummaryTissue extracts from ionizing readiation exposed mouse lung and controls were compared via NMR based metabolomic analysis
Institute
Pacific Northwest National Laboratory
Last NameHu
First NameJianzhi
AddressPacific Northwest National Laboratory, Richland, WA 99352
EmailJianzhi.Hu@pnnl.gov
Phone+1 (509) 371-6544
Submit Date2016-08-15
Raw Data AvailableYes
Raw Data File Type(s)fid
Chear StudyYes
Analysis Type DetailNMR
Release Date2024-12-31
Release Version1
Jianzhi Hu Jianzhi Hu
https://dx.doi.org/10.21228/M8VW2C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000365
Project DOI:doi: 10.21228/M8VW2C
Project Title:NMR based quantitative metabolomics studies of heart and lung from mouse exposed to whole body high dose gamma and proton radiation
Project Summary:Tissue extracts from ionizing readiation exposed mouse heart and controls were compared via NMR based metabolomic analysis
Institute:Pacific Northwest National Laboratory
Department:Fundamental & Computational Sciences
Last Name:Hu
First Name:Jianzhi
Address:Pacific Northwest National Laboratory, Richland, WA 99352
Email:Jianzhi.Hu@pnnl.gov
Phone:(+1)5093716544

Subject:

Subject ID:SU000501
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id days post to radiation radiation dose radiation source
SA024541G31511 3.0 gamma
SA024542G31111 3.0 gamma
SA024543G31411 3.0 gamma
SA024544G31311 3.0 gamma
SA024545G31211 3.0 gamma
SA024546P31111 3.0 proton
SA024547P31211 3.0 proton
SA024548P31511 3.0 proton
SA024549P31411 3.0 proton
SA024550P31311 3.0 proton
SA024537G01311 - sham
SA024538G01411 - sham
SA024539G01211 - sham
SA024540G01111 - sham
SA024555G3444 3.0 gamma
SA024556G3424 3.0 gamma
SA024557G3434 3.0 gamma
SA024558G3454 3.0 gamma
SA024559G3414 3.0 gamma
SA024560G7434 7.8 gamma
SA024561G7444 7.8 gamma
SA024562G7424 7.8 gamma
SA024563G7414 7.8 gamma
SA024551G0424 - sham
SA024552G0434 - sham
SA024553G0444 - sham
SA024554G0414 - sham
Showing results 1 to 27 of 27

Collection:

Collection ID:CO000495
Collection Summary:Lungs were dissected and snap-frozen at -80°C before NMR spectroscopy and morphology analysis.
Sample Type:Lung

Treatment:

Treatment ID:TR000515
Treatment Summary:A total of 27 seven-week-old C57BL/6 female mice randomly into 6 groups, and then whole body being exposed to either gamma or proton radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility.Mice placed into aerated polystyrene boxes immediately before exposure to the entrance region of proton at the BNL Alternating Gradient Synchrotron (AGS) (1055 MeV/nucleon at the target, with a track-averaged LET =148.2 keV/μm at target). Doses of 0 and 3.0 Gy were delivered to a maximum of four mice at ~0.83 Gy/min in a single fraction. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Sample Preparation:

Sampleprep ID:SP000508
Sampleprep Summary:Step 1: After randomization of the samples, every preweighted intact frozen lung tissue was homogenized by a Tissue Tearsor (Model 985-370, BioSpec Product, Inc.) in ice-cold after adding 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Analysis:

Analysis ID:AN000746
Analysis Type:NMR
Num Factors:6
Num Metabolites:58
Units:µM/mg

NMR:

NMR ID:NM000085
Analysis ID:AN000746
Instrument Name:Varian INOVA 600
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:600 MHz
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