Summary of Study ST000500

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000378. The data can be accessed directly via it's Project DOI: 10.21228/M8659W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000500
Study TitleMetabolomics Analysis of Kidney from C57BL/6 Mouse Exposed to Ionizing Radiation
Study TypeTissue Extracts Comparison
Study SummaryTissue extracts from ionizing readiation exposed mouse kidney and controls were compared via NMR based metabolomic analysis
Institute
Pacific Northwest National Laboratory
Last NameHu
First NameJianzhi
Address3335 Innovation Blvd, Richland, Washington, 99354, USA
EmailJianzhi.Hu@pnnl.gov
Phone(509)371-6544
Submit Date2016-10-31
Raw Data File Type(s)fid
Chear StudyYes
Analysis Type DetailNMR
Release Date2024-12-31
Release Version1
Jianzhi Hu Jianzhi Hu
https://dx.doi.org/10.21228/M8659W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000378
Project DOI:doi: 10.21228/M8659W
Project Title:Metabolomics Analysis of Kidney from C57BL/6 Mouse Exposed to Ionizing Radiation
Project Summary:NMR based Metabolomics Analysis of Kidney from C57BL/6 Mouse Exposed to Ionizing Radiation
Institute:Pacific Northwest National Laboratory
Department:Fundamental & Computational Sciences
Last Name:Hu
First Name:Jianzhi
Address:3335 Innovation Blvd, Richland, Washington, 99354, USA
Email:Jianzhi.Hu@pnnl.gov
Phone:(509)371-6544

Subject:

Subject ID:SU000522
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Post Radiation (days) Radiation dose Radiation Source
SA025960G31511 3 gamma
SA025961G31111 3 gamma
SA025962G31411 3 gamma
SA025963G31311 3 gamma
SA025964G31211 3 gamma
SA025965P31111 3 proton
SA025966P31211 3 proton
SA025967P31511 3 proton
SA025968P31411 3 proton
SA025969P31311 3 proton
SA025956G01311 - sham
SA025957G01411 - sham
SA025958G01211 - sham
SA025959G01111 - sham
SA025974G3444 3 gamma
SA025975G3424 3 gamma
SA025976G3434 3 gamma
SA025977G3454 3 gamma
SA025978G3414 3 gamma
SA025979G7434 7.8 gamma
SA025980G7444 7.8 gamma
SA025981G7424 7.8 gamma
SA025982G7414 7.8 gamma
SA025970G0424 - sham
SA025971G0434 - sham
SA025972G0444 - sham
SA025973G0414 - sham
Showing results 1 to 27 of 27

Collection:

Collection ID:CO000516
Collection Summary:Kidneys were dissected and snap-frozen at -80°C before NMR spectroscopy and morphology analysis.
Sample Type:Kidney

Treatment:

Treatment ID:TR000536
Treatment Summary:A total of 27 seven-week-old C57BL/6 female mice randomly into 6 groups, and then whole body being exposed to either gamma or proton radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility.Mice placed into aerated polystyrene boxes immediately before exposure to the entrance region of proton at the BNL Alternating Gradient Synchrotron (AGS) (1055 MeV/nucleon at the target, with a track-averaged LET =148.2 keV/μm at target). Doses of 0 and 3.0 Gy were delivered to a maximum of four mice at ~0.83 Gy/min in a single fraction. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Sample Preparation:

Sampleprep ID:SP000529
Sampleprep Summary:Step 1: After randomization of the samples, every preweighted intact frozen kidney tissue was homogenized by a Tissue Tearsor in ice-cold after adding 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80°C freezer before NMR spectral measurements.

Analysis:

Analysis ID:AN000769
Analysis Type:NMR
Num Factors:6
Num Metabolites:75
Units:µM/mg

NMR:

NMR ID:NM000089
Analysis ID:AN000769
Instrument Name:Varian INOVA 800
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:800 MHz
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