Summary of Study ST000501
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000378. The data can be accessed directly via it's Project DOI: 10.21228/M8659W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000501 |
Study Title | NMR based Metabolomics Analysis of Mouse Exposed to Gamma Radiation |
Study Type | Biofluids Extracts Comparison |
Study Summary | Biofluid extracts from gamma readiation exposed mouse plasma and controls were compared via NMR based metabolomic analysis |
Institute | Pacific Northwest National Laboratory |
Last Name | Hu |
First Name | Jianzhi |
Address | 3335 Innovation Blvd, Richland, Washington, 99354, USA |
Jianzhi.Hu@pnnl.gov | |
Phone | (509)371-6544 |
Submit Date | 2016-11-02 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Chear Study | Yes |
Analysis Type Detail | NMR |
Release Date | 2024-12-31 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000378 |
Project DOI: | doi: 10.21228/M8659W |
Project Title: | Metabolomics Analysis of Kidney from C57BL/6 Mouse Exposed to Ionizing Radiation |
Project Summary: | NMR based Metabolomics Analysis of Kidney from C57BL/6 Mouse Exposed to Ionizing Radiation |
Institute: | Pacific Northwest National Laboratory |
Department: | Fundamental & Computational Sciences |
Last Name: | Hu |
First Name: | Jianzhi |
Address: | 3335 Innovation Blvd, Richland, Washington, 99354, USA |
Email: | Jianzhi.Hu@pnnl.gov |
Phone: | (509)371-6544 |
Subject:
Subject ID: | SU000523 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Radiation dose |
---|---|---|
SA025983 | G041 | - |
SA025984 | G044 | - |
SA025985 | G042 | - |
SA025986 | G043 | - |
SA025987 | G344 | 3.0 |
SA025988 | G345 | 3.0 |
SA025989 | G343 | 3.0 |
SA025990 | G342 | 3.0 |
SA025991 | G341 | 3.0 |
SA025992 | G744 | 7.8 |
SA025993 | G742 | 7.8 |
SA025994 | G741 | 7.8 |
SA025995 | G743 | 7.8 |
Showing results 1 to 13 of 13 |
Collection:
Collection ID: | CO000517 |
Collection Summary: | Plasma was prepared and snap-frozen at -80°C before NMR spectroscopy and morphology analysis. |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000537 |
Treatment Summary: | A total of 13 seven-week-old C57BL/6 female mice randomly into 3 groups, and then whole body being exposed to gamma radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation. |
Sample Preparation:
Sampleprep ID: | SP000530 |
Sampleprep Summary: | Step 1: After randomization of the samples, every preweighted intact frozen plasma was added into MeOH and then vortex 2 mins. After centrifuge at 7200rpm for 5 minutes at 4C, the liqiuds layer in translted into a glass vial. Add 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements. |
Analysis:
Analysis ID: | AN000770 |
Analysis Type: | NMR |
Num Factors: | 3 |
Num Metabolites: | 42 |
Units: | µM/mg |
NMR:
NMR ID: | NM000090 |
Analysis ID: | AN000770 |
Instrument Name: | Varian INOVA 800 |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Spectrometer Frequency: | 800 MHz |