Summary of Study ST000501

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000378. The data can be accessed directly via it's Project DOI: 10.21228/M8659W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000501
Study TitleNMR based Metabolomics Analysis of Mouse Exposed to Gamma Radiation
Study TypeBiofluids Extracts Comparison
Study SummaryBiofluid extracts from gamma readiation exposed mouse plasma and controls were compared via NMR based metabolomic analysis
Institute
Pacific Northwest National Laboratory
Last NameHu
First NameJianzhi
Address3335 Innovation Blvd, Richland, Washington, 99354, USA
EmailJianzhi.Hu@pnnl.gov
Phone(509)371-6544
Submit Date2016-11-02
Raw Data AvailableYes
Raw Data File Type(s)fid
Chear StudyYes
Analysis Type DetailNMR
Release Date2024-12-31
Release Version1
Jianzhi Hu Jianzhi Hu
https://dx.doi.org/10.21228/M8659W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000378
Project DOI:doi: 10.21228/M8659W
Project Title:Metabolomics Analysis of Kidney from C57BL/6 Mouse Exposed to Ionizing Radiation
Project Summary:NMR based Metabolomics Analysis of Kidney from C57BL/6 Mouse Exposed to Ionizing Radiation
Institute:Pacific Northwest National Laboratory
Department:Fundamental & Computational Sciences
Last Name:Hu
First Name:Jianzhi
Address:3335 Innovation Blvd, Richland, Washington, 99354, USA
Email:Jianzhi.Hu@pnnl.gov
Phone:(509)371-6544

Subject:

Subject ID:SU000523
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Radiation dose
SA025983G041-
SA025984G044-
SA025985G042-
SA025986G043-
SA025987G3443.0
SA025988G3453.0
SA025989G3433.0
SA025990G3423.0
SA025991G3413.0
SA025992G7447.8
SA025993G7427.8
SA025994G7417.8
SA025995G7437.8
Showing results 1 to 13 of 13

Collection:

Collection ID:CO000517
Collection Summary:Plasma was prepared and snap-frozen at -80°C before NMR spectroscopy and morphology analysis.
Sample Type:Blood

Treatment:

Treatment ID:TR000537
Treatment Summary:A total of 13 seven-week-old C57BL/6 female mice randomly into 3 groups, and then whole body being exposed to gamma radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Sample Preparation:

Sampleprep ID:SP000530
Sampleprep Summary:Step 1: After randomization of the samples, every preweighted intact frozen plasma was added into MeOH and then vortex 2 mins. After centrifuge at 7200rpm for 5 minutes at 4C, the liqiuds layer in translted into a glass vial. Add 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Analysis:

Analysis ID:AN000770
Analysis Type:NMR
Num Factors:3
Num Metabolites:42
Units:µM/mg

NMR:

NMR ID:NM000090
Analysis ID:AN000770
Instrument Name:Varian INOVA 800
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:800 MHz
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