Summary of Study ST000506

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000371. The data can be accessed directly via it's Project DOI: 10.21228/M83C78 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000506
Study TitleTimecourse of TCA panel in LNCaP Cells
Study SummaryTimecourse of TCA panel in LNCaP Cells
Institute
Mayo Clinic
Last NameFrigo
First NameDaniel
AddressDepartment of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001
Emailfrigo@uh.edu
Phone832-842-8824
Submit Date2016-10-21
Analysis Type DetailGC-MS
Release Date2018-12-11
Release Version1
Daniel Frigo Daniel Frigo
https://dx.doi.org/10.21228/M83C78
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000371
Project DOI:doi: 10.21228/M83C78
Project Title:Metabolic Dissection of Aberrant Glutamine Metabolism in Prostate Cancer
Project Type:MS targeted analysis
Project Summary:In this project, we propose to define the specific role(s) of three crucial oncogenic signaling cascades (androgen receptor, Myc and AMPK) in regulating glutamine metabolism in prostate cancer using 13C-labeled glutamine with mass spectrometry-based metabolic flux analyses.
Institute:Mayo Clinic
Laboratory:Mayo Clinic Metabolomics Resource Core
Last Name:Frigo
First Name:Daniel
Address:Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001
Email:frigo@uh.edu
Phone:832-842-8824

Subject:

Subject ID:SU000528
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA026280sample # 9100 pM R1881
SA026281sample # 6100 pM R1881
SA026282sample # 2100 pM R1881
SA026283sample # 13100 pM R1881
SA026276sample # 1410 nM R1881
SA026277sample # 710 nM R1881
SA026278sample # 410 nM R1881
SA026279sample # 1110 nM R1881
SA026274sample # 101 nM R1881
SA026275sample # 31 nM R1881
SA026284sample # 8vehicle (ethanol)
SA026285sample # 5vehicle (ethanol)
SA026286sample # 1vehicle (ethanol)
SA026287sample # 12vehicle (ethanol)
Showing results 1 to 14 of 14

Collection:

Collection ID:CO000522
Collection Summary:LNCaP media
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000542
Treatment Summary:LNCaP cells that were serum starved and incubated with 2mM [U-13C]glutamine and treated with dose response of a synthetic androgen (R1881) for 24 or 72 hours.

Sample Preparation:

Sampleprep ID:SP000535
Sampleprep Summary:TCA media concentrations

Combined analysis:

Analysis ID AN000775
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent HP5-MS (30m × 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units nmol/ml

Chromatography:

Chromatography ID:CH000555
Instrument Name:Agilent 7890B
Column Name:Agilent HP5-MS (30m × 0.25mm, 0.25 um)
Chromatography Type:GC

MS:

MS ID:MS000682
Analysis ID:AN000775
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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