Summary of Study ST000532

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000391. The data can be accessed directly via it's Project DOI: 10.21228/M8HG71 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000532
Study TitleCHEAR Plasma Reference Material Plasma GC-MS
Study TypeMetabolomics
Study SummaryCHEAR Reference Material Plasma was provided by Emory University. The material was prepared and analyzed using the GC-TOF MS workflow of the Eastern Regional Metabolomics Resource Core (protocols available in metabolomics workbench). Seven samples were injected of the sample reference material prepared in replicate.
Institute
RTI International
DepartmentDiscovery-Sciences-Technology (DST)
LaboratoryRTI CHEAR Analytical Hub - Untargeted Analysis Resource Core
Last NameFennell
First NameTimothy
Address3040 E Cornwallis Road, Research Triangle Park, NC 27709
Emailfennell@rti.org
Phone919-485-2781
Submit Date2016-12-30
Raw Data AvailableYes
Raw Data File Type(s)cdf
Chear StudyYes
Analysis Type DetailGC-MS
Release Date2024-12-31
Release Version1
Timothy Fennell Timothy Fennell
https://dx.doi.org/10.21228/M8HG71
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000391
Project DOI:doi: 10.21228/M8HG71
Project Title:CHEAR Plasma Reference Material ProficiencyTest GC-MS
Project Type:CHEAR-UT-1
Project Summary:CHEAR Reference Material Plasma was provided by Emory University. The material was prepared and analyzed using the GC-TOF MS workflow of the Eastern Regional Metabolomics Resource Core (protocols available in metabolomics workbench). Seven samples were injected of the sample reference material prepared in replicate.
Institute:RTI International
Department:Discovery-Science-Technology
Laboratory:RTI CHEAR Analytical Hub - Untargeted Analysis Resource Core (UARC)
Last Name:Fennell
First Name:Timothy
Address:3040 E Cornwallis Road, Research Triangle Park, NC 27709
Email:fennell@rti.org
Phone:919-485-2781

Subject:

Subject ID:SU000554
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id SAMPLE TYPE
SA027902CHE000054 0207aReplicate
SA027903CHE000054 0208aReplicate
SA027904CHE000054 0206aReplicate
SA027905CHE000054 0205aReplicate
SA027906CHE000054 0203aReplicate
SA027907CHE000054 0204aReplicate
SA027908CHE000054 0202aReplicate
Showing results 1 to 7 of 7

Collection:

Collection ID:CO000548
Collection Summary:CHEAR Reference Material Plasma was provided by Emory University.
Sample Type:Blood

Treatment:

Treatment ID:TR000568
Treatment Summary:No treatment.

Sample Preparation:

Sampleprep ID:SP000561
Sampleprep Summary:liquots of 30µL of plasma were mixed with 1000µL of (3:3:2) ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Samples resuspended in 450µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Combined analysis:

Analysis ID AN000811
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus 4D GC
Column Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units intensity

Chromatography:

Chromatography ID:CH000584
Instrument Name:Leco Pegasus 4D GC
Column Name:Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Column Pressure:10.4 psi
Flow Gradient:Constant Flow
Flow Rate:1mL/min
Injection Temperature:250 degrees C
Retention Index:FAME retention index markers C8-C30
Sample Injection:0.5uL
Analytical Time:20 minutes
Transferline Temperature:280 degrees C
Chromatography Type:GC

MS:

MS ID:MS000718
Analysis ID:AN000811
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
Ion Mode:POSITIVE
  logo