Summary of study ST000556

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000407. The data can be accessed directly via it's Project DOI: 10.21228/M8FG7D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000556
Study TitleIROA feasibility project; plasticizers as obesogens in zebrafish
Study Type(1) IROA label (2) Zebrafish exposed to DEHP
Study SummaryZebrafish were fed IROA labelled nematodes (smaple 1-4); In a second experiment, zebrafish larvae were exposed to DEHP, a chemical that is a suspected obesogen.
Institute
University of Florida
DepartmentSECIM
Last NameMartyniuk
First NameChris
Address2187 Mowry Rd. Bldg 471
Emailcmartyn@ufl.edu
Phone352-294-4636
Submit Date2017-01-26
Num Groups2
Total Subjects20
Study CommentsExpt 2: Two groups (control) + DEHP-treated (n=10 larvae pools or biological replicates)
Raw Data AvailableYes
Raw Data File Type(s).mzML
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Chris Martyniuk Chris Martyniuk
https://dx.doi.org/10.21228/M8FG7D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000407
Project DOI:doi: 10.21228/M8FG7D
Project Title:IROA feasibility project; plasticizers as obesogens in zebrafish
Project Summary:IROA feasibility project; plasticizers as obesogens in zebrafish
Institute:University of Florida
Department:Physiological Sciences
Last Name:Martyniuk
First Name:Chris
Address:2187 Mowry Rd. Bldg 471
Email:cmartyn@ufl.edu
Phone:352-294-4636
Funding Source:"Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209 "

Subject:

Subject ID:SU000578
Subject Type:Larval fish at one month
Subject Species:Danio rerio
Taxonomy ID:7955
Species Group:Fish

Factors:

Subject type: Larval fish at one month; Subject species: Danio rerio (Factor headings shown in green)

mb_sample_id local_sample_id Chemical Treatment
SA028761M18ADEHP
SA028762M20ADEHP
SA028763M1ADEHP
SA028764M12ADEHP
SA028765M15ADEHP
SA028766M11ADEHP
SA028767M2ADEHP
SA028768M5ADEHP
SA028769M3ADEHP
SA028770M7ADEHP
SA028771C13ADMSO
SA028772C15ADMSO
SA028773C18ADMSO
SA028774C12ADMSO
SA028775C2ADMSO
SA028776C3ADMSO
SA028777C4ADMSO
SA028778C5ADMSO
SA028779C9ADMSO
SA028780C10ADMSO
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000572
Collection Summary:Expt 2: Larval zebrafish were exposed to a plastic for 96 hours. Fish were pooled into groups of 5 (10 biological replicates derived from 10 independent technical replicates.
Sample Type:Whole larval fish.

Treatment:

Treatment ID:TR000592
Treatment Summary:Expt 2: Larval zebrafish were exposed to a plastic for 96 hours.
Treatment Compound:DEHP (a plastic)
Treatment Route:waterborne
Treatment Dose:10 uM DEHP
Treatment Doseduration:96 hours
Treatment Vehicle:DMSO
Animal Vet Treatments:None
Animal Anesthesia:Tricaine
Animal Fasting:Early stage larval fish, do not feed for first 144 hours.
Animal Endp Euthanasia:MS-222 or Tricaine
Animal Endp Tissue Coll List:Whole larval fish placed into a vial. Five fish per vial.
Animal Endp Clinical Signs:No clinical signs

Sample Preparation:

Sampleprep ID:SP000585
Sampleprep Summary:None
Sampleprep Protocol Filename:Metabolomics_Global_LCMS.pdf
Metabolomics_LCMSProtocol_plasma.pdf

Combined analysis:

Analysis ID AN000853 AN000854
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000608
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000754
Analysis ID:AN000853
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000755
Analysis ID:AN000854
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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