Summary of study ST000588

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000430. The data can be accessed directly via it's Project DOI: 10.21228/M8GC82 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000588
Study TitleMetabolomics of Saliva in Decompensated Heart Failure
Study SummaryThe aim of the study was to test weather characteristic differences or changes in metabolic profile exist between exhaled breath condensate (EBC) and saliva of healthy individuals and heart failure patients. Salival NMR profiling was performed.
Institute
Mayo Clinic
DepartmentCardiovascular Diseases
LaboratoryMayo Metabolomics Core
Last NameJohnson
First NameBruce
Address200 First Street SW, Rochester, MN 55905
Emailjohnson.bruce@mayo.edu
Phone507-284-4441
Submit Date2017-04-07
Analysis Type DetailNMR
Release Date2019-05-15
Release Version1
Bruce Johnson Bruce Johnson
https://dx.doi.org/10.21228/M8GC82
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000430
Project DOI:doi: 10.21228/M8GC82
Project Title:Mayo Metabolomics Pilot and Feasibility Award: Metabolomics of Exhaled Breath Condensate in Decompensated Heart Failure
Project Summary:Metabolomics is a large-scale approach to monitoring as many as possible of the compounds involved in cellular processes in a single assay to derive metabolic profiles. Compared with genomics or proteomics, metabolomics reflects changes in phenotype and therefore function. Nearly all the blood volume-cardiac output in humans flows through the lungs at a rate of 5L/min and traverses through capillaries with a very thin blood-gas barrier (approximately 2μm, 600–800 nm; in some places <200 nm) to allow sufficient gas diffusion. However, there is evidence that many other biochemicals from the blood can be found in the exhaled air. Collection of exhaled breath condensate (EBC) is a relatively new, noninvasive method that allows assessment of biochemical profiles that may not only link to airway physiology, but also to other changing processes in the body. The aims of this study are: (1) Working with the Mayo Clinic Metabolomics Resource Core Laboratory to develop the optimal method for performing metabolomics of EBC, (2) Collecting and metabolomic profiling of the EBCs from patients with heart failure; (2) Comparing the profiles of these patients obtained during stable and acute exacerbation periods as well as during recovery from a decompensation; (3) Comparing the profiles of these patients with age and gender matched controls; (4) Correlation of EBC metabolomic profiles with clinical phenotypes. The proposed metabolomics profiles would be obtained from patients involved in IRB approved, ongoing, primarily NIH funded studies focused on understanding pulmonary congestion in heart failure patients. This includes a recently started project capturing patients as they present in the emergency room with decompensated heart failure. Phenotyping for this study includes novel measures of pulmonary capillary recruitment, alveolar-capillary conductance, pulmonary capillary blood volume, thoracic CT imaging combined with in-hospital clinical data, including blood biomarkers such as BNP and catecholamines. Measurements are performed upon admission (within 24hours) and then repeated just prior to dismissal. In addition, we would collect EBC on stable HF patients and in age and gender matched controls healthy controls from our other ongoing studies. The overall aim will be to identify novel patterns or biomarkers for developing a point-of-care test (POC) for tracking disease status in HF patients.
Institute:Mayo Clinic
Department:Cardiovascular Diseases
Laboratory:Mayo Metabolomics Core
Last Name:Johnson
First Name:Bruce
Address:200 First St. SW, Rochester, Minnesota, 55905, USA
Email:johnson.bruce@mayo.edu
Phone:507-284-4441

Subject:

Subject ID:SU000611
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA031841C22Control
SA031842C23Control
SA031843C10Control
SA031844C20Control
SA031845C24Control
SA031846C21Control
SA031847C19Control
SA031848C11Control
SA031849C15Control
SA031850C14Control
SA031851C16Control
SA031852C18Control
SA031853HF12Heart failure
SA031854HF09Heart failure
SA031855HF17Heart failure
SA031856HF08Heart failure
SA031857HF13Heart failure
SA031858HF01Heart failure
SA031859HF03Heart failure
SA031860HF02Heart failure
SA031861HF04Heart failure
SA031862HF05Heart failure
SA031863HF06Heart failure
SA031864HF07Heart failure
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000605
Collection Summary:The samples were collected in Dr. Bruce Johnson lab, at the Department of Cardiovascular Diseases, Mayo Clinic. EBC samples were collected using Jaeger EcoScreen cooling unit (-20°C). Patients were breathing through mouth piece while wearing a nose clip during 30 minutes. Saliva was collected from the same patients during last 15 minutes. The samples were frozen immediately after collection.
Sample Type:Saliva

Treatment:

Treatment ID:TR000625
Treatment Summary:We will focus initially on patients with classic systolic HF who are admitted to St Mary’s Hospital with decompensated disease. These patients are currently being studied to quantify changes in lung water during the “recompensation” diuresis in the hospital and the related changes in alveolar gas conductance and breathing pattern to develop a predictive algorithm for likelihood of readmission, but also to obtain a better understanding of fluid regulation in the lungs. As these subjects are recruited, we will recruit age and sex matched stable HF patients going through similar testing in parallel running studies as well as age and sex matched control subjects. We will target 12 subjects per group and in addition to the EBC collection we will obtain saliva samples and blood at the same time points.

Sample Preparation:

Sampleprep ID:SP000618
Sampleprep Summary:Saliva: Allow samples to thaw on ice at 4 °C for 30–60 min. Transfer 250 μl aliquots of saliva samples into 1.5ml EP tubes, centrifuge each sample at 13,300g for 10 min. Transfer 200μl of supernatant of saliva samples into 1.5 ml EP tubes, add 150 μl of phosphate buffer and 150 μl of 4mM TSP-d4 solution in D2O. Vortex for 20 s. Transfer the solution to 5 mm NMR tube.

Analysis:

Analysis ID:AN000903
Analysis Type:NMR
Num Factors:2
Num Metabolites:48
Units:uM

NMR:

NMR ID:NM000101
Analysis ID:AN000903
Instrument Name:Bruker 600 MHz Avance III HD spectrometer
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:600 MHz
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