Summary of study ST000667

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000470. The data can be accessed directly via it's Project DOI: 10.21228/M89G75 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000667
Study TitleDiabetic Microvascular Plasma/Tissue Lipidomics- Comparison
Study TypeMS analysis
Study SummaryDetermining lipid composition of diabetic microvascular complication-prone tissues and comparing tissues levels to plasma levels. Samples are in addition to plasma and kidney tissue samples ran (shotgun lipidomics) in June-July 2014.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups6
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M89G75
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000470
Project DOI:doi: 10.21228/M89G75
Project Title:Diabetic Microvascular Plasma/Tissue Lipidomics- Comparison
Project Type:MS analysis
Project Summary:Determine lipids in plasma and microvascular complication prone tissues in diabetic mouse models
Institute:University of Michigan
Department:UMich Internal Medicine
Laboratory:Pennathur Lab
Last Name:Pennathur
First Name:Subramaniam
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Email:spennath@umich.edu
Phone:734 615 5757

Subject:

Subject ID:SU000690
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id CONTROL_DIABETIC Sample type
SA038424S00018519db/db diabetic Kidney
SA038425S00018520db/db diabetic Kidney
SA038426S00018521db/db diabetic Kidney
SA038427S00018523db/db diabetic Plasma
SA038428S00018525db/db diabetic Plasma
SA038429S00018522db/db diabetic Plasma
SA038430S00018524db/db diabetic Plasma
SA038431S00018516db/db diabetic Retina
SA038432S00018517db/db diabetic Retina
SA038433S00018515db/db diabetic Retina
SA038434S00018514db/db diabetic Retina
SA038435S00018508db/db diabetic Sciatic Nerve
SA038436S00018504db/db diabetic Sciatic Nerve
SA038437S00018505db/db diabetic Sciatic Nerve
SA038438S00018506db/db diabetic Sciatic Nerve
SA038439S00018507db/db diabetic Sciatic Nerve
SA038440S00018518db/m control Kidney
SA038441S00018526db/m control Plasma
SA038442S00018513db/m control Retina
SA038443S00018510db/m control Retina
SA038444S00018511db/m control Retina
SA038445S00018509db/m control Retina
SA038446S00018512db/m control Retina
SA038447S00018500db/m control Sciatic Nerve
SA038448S00018501db/m control Sciatic Nerve
SA038449S00018503db/m control Sciatic Nerve
SA038450S00018499db/m control Sciatic Nerve
SA038451S00018502db/m control Sciatic Nerve
Showing results 1 to 28 of 28

Collection:

Collection ID:CO000684
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000704
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000697
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001020 AN001021
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000730
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000915
Analysis ID:AN001020
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000916
Analysis ID:AN001021
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
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