Summary of study ST000674

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000476. The data can be accessed directly via it's Project DOI: 10.21228/M8J02M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download all metabolite data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data (Contains raw data)
Study IDST000674
Study TitleBmal1-ethanol based diet and liver injury
Study TypeMS analysis
Study SummaryThe mice were injected via tail vein with either shBmal1 adenovirus or shLacz adenocirus as control (n=5 for each group), following 10 days of 5% ethanol diet feeding. Then the mice were dissected and liver tissues were flash freezed. 25mg liver tissues from each mouse of the same group were pooled.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups2
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8J02M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000476
Project DOI:doi: 10.21228/M8J02M
Project Title:Bmal1-ethanol based diet and liver injury
Project Type:MS analysis
Project Summary:We want to test whether Bmal1 deficiency sensitizes ethanol induced liver injury
Institute:University of Michigan
Department:Molecular & Integrative Physiology
Laboratory:Yin Lab
Last Name:Yin
First Name:Lei
Address:Ann Arbor, MI
Email:leiyin@umich.edu
Phone:734-764-9920

Subject:

Subject ID:SU000697
Subject Type:Mouse
Subject Species:Mus
Taxonomy ID:10088
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus (Factor headings shown in green)

mb_sample_id local_sample_id Type
SA038669S00022594Bmal1 knockdown
SA038670S00022593control
Showing results 1 to 2 of 2

Collection:

Collection ID:CO000691
Collection Summary:-
Sample Type:Liver

Treatment:

Treatment ID:TR000711
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000704
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001034 AN001035
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000737
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000929
Analysis ID:AN001034
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000930
Analysis ID:AN001035
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
  logo