Summary of Study ST000674
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000476. The data can be accessed directly via it's Project DOI: 10.21228/M8J02M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000674 |
| Study Title | Bmal1-ethanol based diet and liver injury |
| Study Type | MS analysis |
| Study Summary | The mice were injected via tail vein with either shBmal1 adenovirus or shLacz adenocirus as control (n=5 for each group), following 10 days of 5% ethanol diet feeding. Then the mice were dissected and liver tissues were flash freezed. 25mg liver tissues from each mouse of the same group were pooled. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 2 |
| Total Subjects | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000476 |
| Project DOI: | doi: 10.21228/M8J02M |
| Project Title: | Bmal1-ethanol based diet and liver injury |
| Project Type: | MS analysis |
| Project Summary: | We want to test whether Bmal1 deficiency sensitizes ethanol induced liver injury |
| Institute: | University of Michigan |
| Department: | Molecular & Integrative Physiology |
| Laboratory: | Yin Lab |
| Last Name: | Yin |
| First Name: | Lei |
| Address: | Ann Arbor, MI |
| Email: | leiyin@umich.edu |
| Phone: | 734-764-9920 |
Subject:
| Subject ID: | SU000697 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Type |
|---|---|---|
| SA038669 | S00022594 | Bmal1 knockdown |
| SA038670 | S00022593 | control |
| Showing results 1 to 2 of 2 |
Collection:
| Collection ID: | CO000691 |
| Collection Summary: | - |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000711 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000704 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001034 | AN001035 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000737 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000929 |
| Analysis ID: | AN001034 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000930 |
| Analysis ID: | AN001035 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
