Summary of study ST000685

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000484. The data can be accessed directly via it's Project DOI: 10.21228/M8H03P This work is supported by NIH grant, U2C- DK119886.

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Study IDST000685
Study TitleLipidomics analysis for human liver cell lines
Study TypeMS analysis
Study SummaryOn day 1, seed 1 million cells/dish Huh7_Lok cells (vector, GCKR(WT), GCKR(P446L), Non-Target, GCKR KD and PPP1R3B/OE) in 100 mm dishes with DMEM complete medium and incubate at 37C for 24 h. On day 2, replace DMEM medium with delipidated serum medium for these cells and incubate for another 24 h. On day 3, add heated Oleic Acid-Albumin into each dish at final concentration of 200?M for 24h. On day 4, collect cells with Trypsin/EDTA lysis and count cells with automated cell counter and calculate total cell numbers in each sample, then centrifuge cells down and take out the supernatant and keep cells in -80 C freezer.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups6
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8H03P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000484
Project DOI:doi: 10.21228/M8H03P
Project Title:Molecular genetic study on nonalcoholic fatty liver disease (NAFLD)
Project Type:MS analysis
Project Summary:In this study, we determine the genetic mechanism of action by which variants near GCKR and PPP1R3B exert their effects on promoting NAFLD
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Speliotes Lab
Last Name:Speliotes
First Name:Elizabeth
Address:Ann Arbor, MI
Email:espeliot@umich.edu
Phone:734-647-2964

Subject:

Subject ID:SU000708
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Construct
SA039079S00027766GCKR_52621
SA039080S00027767GCKR_52621
SA039081S00027768GCKR_52621
SA039073S00027759GCKR(P446L)
SA039074S00027758GCKR(P446L)
SA039075S00027760GCKR(P446L)
SA039076S00027756GCKR(WT)
SA039077S00027755GCKR(WT)
SA039078S00027757GCKR(WT)
SA039082S00027764NT
SA039083S00027763NT
SA039084S00027765NT
SA039085S00027762PPP1R3B
SA039086S00027761PPP1R3B
SA039087S00027753Vector
SA039088S00027752Vector
SA039089S00027754Vector
Showing results 1 to 17 of 17

Collection:

Collection ID:CO000702
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000722
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000715
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001056 AN001057
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000748
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000951
Analysis ID:AN001056
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000952
Analysis ID:AN001057
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
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