Summary of study ST000741

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000535. The data can be accessed directly via it's Project DOI: 10.21228/M8HH47 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000741
Study TitleMetabolite-phenotype link in X-linked Adrenoleukodystrophy (fibroblast cell culture)
Study TypeMS analysis
Study SummaryUntargeted metabolomics of control, AMN and ALD patient-derived fibroblasts. Fibroblasts cultured in DMEM with 15%FBS and antibiotic (pen/strep) in 100mm petridishes (1million/dish). After 24h cultures supplemented with fresh media (DMEM+15%FBS+antibiotic) for 3hr
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HH47
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000535
Project DOI:doi: 10.21228/M8HH47
Project Title:Metabolite-phenotype link in X-linked Adrenoleukodystrophy
Project Type:MS analysis
Project Summary:Untargeted metabolomics of control, AMN and ALD patient-derived fibroblasts
Institute:Henry Ford Health System
Department:Neurology
Laboratory:Singh Lab
Last Name:Singh
First Name:Jaspreet
Address:Detroit, MI
Email:jsingh2@hfhs.org
Phone:313-916-7725

Subject:

Subject ID:SU000764
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id subject type
SA041168S00016575Healthy control
SA041169S00016570Healthy control
SA041170S00016574Healthy control
SA041171S00016573Healthy control
SA041172S00016571Healthy control
SA041173S00016572Healthy control
SA041174S00016580Mild disease
SA041175S00016581Mild disease
SA041176S00016579Mild disease
SA041177S00016578Mild disease
SA041178S00016576Mild disease
SA041179S00016577Mild disease
SA041180S00017166POOLED
SA041181S00016587Severe disease
SA041182S00016586Severe disease
SA041183S00016584Severe disease
SA041184S00016582Severe disease
SA041185S00016583Severe disease
SA041186S00016585Severe disease
Showing results 1 to 19 of 19

Collection:

Collection ID:CO000758
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000778
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000771
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001155 AN001156
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000815
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001050
Analysis ID:AN001155
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001051
Analysis ID:AN001156
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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