Summary of study ST000752

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000542. The data can be accessed directly via it's Project DOI: 10.21228/M8M96V This work is supported by NIH grant, U2C- DK119886.

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Study IDST000752
Study TitleEffect of cooling on muscle tissue metabolism
Study TypeMS analysis
Study SummaryThermal modalities are commonly used in sports medicine to affect tissue healing. Cold therapy is commonly used modalities, but the metabolic changes to muscle after cooling are not known. The objective of this study is to look at the effect of cooling on muscle metabolites and gene expression. There are a total of 8 subjects in the study. Each subject had an ice cup cryotherapy treatment ("cool" samples) to one leg for 15 minutes, and the other leg served as the control ("cntrl" samples). Two hours after the application of cryotherapy, a biopsy was taken from each thigh muscle. Muscle was minced with scissors and quickly snap frozen in liquid nitrogen.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups10
Total Subjects8
Study CommentsWe would like to run the TCA Plus Analysis on each of the samples. For the statistical analysis, we would normalize the cool side to the ctrl side, and look at the relative change in metabolite abundance as a result of the cooling procedure. only used in sports medicine to affect tissue healing.
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8M96V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000542
Project DOI:doi: 10.21228/M8M96V
Project Title:Thermal Modalities Study
Project Type:MS analysis
Project Summary:This study is looking at the effect of cooling on muscle tissue metabolism
Institute:University of Michigan
Department:Orthopaedic Surgery
Laboratory:Mendias Lab
Last Name:Mendias
First Name:Christopher
Address:Ann Arbor, MI
Email:cmendias@umich.edu
Phone:734-764-3250

Subject:

Subject ID:SU000775
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Temperature
SA041468S00023215Control
SA041469S00023225Control
SA041470S00023229Control
SA041471S00023221Control
SA041472S00023227Control
SA041473S00023223Control
SA041474S00023217Control
SA041475S00023219Control
SA041476S00023230Cooled
SA041477S00023216Cooled
SA041478S00023228Cooled
SA041479S00023226Cooled
SA041480S00023220Cooled
SA041481S00023224Cooled
SA041482S00023222Cooled
SA041483S00023218Cooled
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000769
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000789
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000782
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_-_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001180
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent
Column Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520B QTOF
Ion Mode NEGATIVE
Units nmol/mg

Chromatography:

Chromatography ID:CH000828
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001075
Analysis ID:AN001180
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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