Summary of study ST000756

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000545. The data can be accessed directly via it's Project DOI: 10.21228/M87083 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000756
Study TitleMetabolomics of cilia and modulation of renal microcirculation
Study TypeMS analysis
Study SummaryExplore and explain the mechanism of regulation of metabolism biomarkers to modulate renal microcirculation. 1 - let rats rest for 5 days. 2 - test basal glucose and plasma. Store plasma in -20C for one day then transfer to -80. 3 - inject STZ 50mg/kg ip. 4 - after 3 days, check for increased glucose and extract plasma for early stage diabetic sample >300 mg/dl. Store in -20C and then -80C. 5 - one month after initial injection, extract plasma and store in -20C and then -80C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups6
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M87083
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000545
Project DOI:doi: 10.21228/M87083
Project Title:Cilia and Modulation of Renal Microcirculation
Project Type:MS analysis
Project Summary:Explore and explain the mechanism of regulation of metabolism biomarkers to modulate renal microcirculation
Institute:University of South Florida
Department:Molecular Pharmacology & Physiology
Laboratory:Liu Lab
Last Name:Liu
First Name:Ruisheng
Address:Tampa, FL
Email:ruisheng@health.usf.edu
Phone:813-396-9107

Subject:

Subject ID:SU000779
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10114
Species Group:Mammal

Factors:

Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Days post-STZ
SA041592S00016983-
SA041593S00016982-
SA041594S00016981-
SA041595S000169863
SA041596S000169853
SA041597S000169843
SA041598S0001698930
SA041599S0001698830
SA041600S0001698730
SA041601S00017328POOLED
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000773
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000793
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000786
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001186 AN001187
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000833
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001081
Analysis ID:AN001186
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001082
Analysis ID:AN001187
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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