Summary of study ST000768

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000554. The data can be accessed directly via it's Project DOI: 10.21228/M8295H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000768
Study TitleMetabolites of human neurons and stem cells (siNeuron metabolomics)
Study TypeMS analysis
Study SummaryThere are a total of four samples each for this analysis. Each sample consists of the cells grown on three 10 cm culture plates. Each plate should have 2x106 cells for a total of 6x106 cells per sample when all three plates are combined. The first sample is undifferentiated human embryonic stem cells, the second sample is human glutamatergic neurons derived from those human embryonic stem cells, the third sample is undifferentiated human induced pluripotent stem cells and the fourth sample is human glutamatergic neurons derived from those human induced pluripotent stem cells.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s).D
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8295H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000554
Project DOI:doi: 10.21228/M8295H
Project Title:Metabolites of human neurons and stem cells (siNeuron metabolomics)
Project Type:MS analysis
Project Summary:metabolites of human neurons and stem cells
Institute:University of Michigan
Department:Mol Behav Neurosci Inst
Laboratory:Uhler Lab
Last Name:Uhler
First Name:Michael
Address:Ann Arbor, MI
Email:muhler@umich.edu
Phone:734-657-3192

Subject:

Subject ID:SU000791
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Media Type
SA042085S000262113N media neurons
SA042086S000262133N media neurons
SA042087S00026212E8 media stem cells
SA042088S00026210E8 media stem cells
Showing results 1 to 4 of 4

Collection:

Collection ID:CO000785
Collection Summary:-
Sample Type:Stem cells

Treatment:

Treatment ID:TR000805
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000798
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001214 AN001215
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000851
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001109
Analysis ID:AN001214
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001110
Analysis ID:AN001215
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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